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Chemokines

Handel, Tracy M., editor.

Title

Chemokines

Author

Handel, Tracy M., editor.

ISBN

9780080956978

9780080956961

9780123749079

9780128021958

Edition

1st ed.

Physical Description

1 online resource (parts A-B) : illustrations (color illustrations)

Series

Methods in enzymology, v. 460, 461

Methods in enzymology ; v. 460.

Methods in enzymology ; v. 461.

Contents

Part A (v. 460) -- Part B (v. 461).

Front Cover; Methods In Enzymology: Chemokines, Part A; Copyright Page; Contents; Contributors; Preface; Section 1: Chemokines and Receptors in Disease; Chapter 1: Chapter One Chemokines in Human Breast Tumor Cells: Modifying Their Expression Levels and Determining Their Effects ; 1. Introduction; 2. Modifying Chemokine Expression in Breast Tumor Cells; 3. Establishment of Primary Local Breast Tumors and Pulmonary Metastases; Acknowledgments; References; Chapter 2: Chapter 2 CCR5 Pharmacology Methodologies and Associated Applications; 1. Introduction.

2. CCR5 Signaling Assays and Application to Quantify and Characterize Ligand-Dependent Agonism, Antagonism, and Inverse Ag3. CCR5-Associated Ligand-Binding Assays; 4. Surrogate In Vitro Antiviral Assays; 5. CCR5 Site-Directed Mutagenesis and Ligand Docking Studies; 6. Non-HIV Indications-Associated Studies, Human CCR5 Knock-In Mice; References; Chapter 3: Chapter Three CXCR4 and Mobilization of Hematopoietic Precursors; 1. Introduction; 2. HSPC Mobilizing Agents that Target the CXCL12/CXCR4 Axis; 3. Donor Selection for HSPC Mobilization; 4. Flow Cytometric Enumeration of Mobilized HSPCs.

5. Dosing and Kinetics of HSPC Mobilization by G-CSF and Plerixafor6. Flow Cytometric Analysis of CXCR4 Expression on Human CD34+ Subsets; 7. Functional Characterization of Mobilized HSPCs; 8. Concluding Remarks; Acknowledgment; References; Chapter 4: Double-Label Nonradioactive ; 1. Introduction; 2. Basic Protocol for ISH (Using Digoxygenin-Labeled Probe); 3. Comments; References; Chapter 5: Chapter Five Expression of Chemokines and Chemokine Receptors in Human Colon Cancer; 1. Introduction; 2. Materials and Methods; 3. Results; 4. Discussion; Acknowledgments; References.

Section II: Chemokine Related Proteins from PathogensChapter 6: Kaposi's Sarcoma Virally Encoded, G-Protein-Coupled Receptor: A Paradigm for Paracrine Transformation; 1. Introduction; 2. Cloning of vGPCR; 3. Assaying vGPCR Transforming Activity In Vitro; 4. vGPCR Transforming Activity In Vivo Using Xenograft Systems; 5. In Vivo Targeted Infection Using the TVA-RCAS System; 6. vGPCR-Induced Paracrine Transformation; 7. Characterization of vGPCR-Induced Molecular Signaling; 8. Evaluation of Activation of Second-Messenger-Generating Systems.

9. Activation of Signal-Transducing Protein Kinases and Small GTPases10. Akt Kinase Assay; 11. vGPCR Stimulated Activation of Rac1-Pulldown Assays; 12. Western Blotting Using Phospho-Specific Antibodies; 13. Activation of Transcription Factors; 14. Global Changes in Gene Expression: Microarray Analysis; 15. NFkappaB Luciferase Assays; 16. NFkappaB Binding Assays; 17. Nuclear Translocation of NFkappaB; Acknowledgments; References; Chapter 7: Chapter 7 Pharmacological and Biochemical Characterization of Human Cytomegalovirus-Encoded G Protein-Coupled Recept; 1. Introduction.

Abstract

The understanding of chemokines, the proteins that control the migration of cells, and their receptors, is critical to the study of causes and therapies for a wide range of human diseases and infections, including certain types of cancer, inflammatory diseases, HIV, and malaria. This volume, focusing on chemokine structure and function, as well as signaling, and its companion volume (Methods in Enzymology volume 461, focusing chemokines as potential targets for disease intervention) provide a comprehensive overview and time-tested protocols in this field, making it an essential reference for researchers in the area. Along with its companion volume, provides a comprehensive overview of chemokine methods, specifically as related to potential disease therapy Gathers tried, tested, and trusted methods and techniques from top players in chemokine research Provides an essential reference on for researchers in the field.

Subject Term

Chemokines.

Chemokines -- Physiological effect.

Chemokines. (DNLM)D018925

HEALTH & FITNESS -- Diseases -- General.

MEDICAL -- Clinical Medicine.

MEDICAL -- Diseases.

MEDICAL -- Evidence-Based Medicine.

MEDICAL -- Internal Medicine.

Chemokines. (OCoLC)fst00853583

Genre

Electronic books.