Caenorhabditis elegans : modern biological analysis of an organism
by
Epstein, Henry F., 1944- editor.
Title
:
Caenorhabditis elegans : modern biological analysis of an organism
Author
:
Epstein, Henry F., 1944- editor.
ISBN
:
9780080859460
9780125641494
Physical Description
:
1 online resource (xxi, 659 pages) : illustrations
Series
:
Methods in cell biology, v. 48
Methods in cell biology ; v. 48.
General Note
:
"Prepared under the auspices of the American Society for Cell Biology."
Contents
:
pt. 1. Genetic and culture methods. Basic culture methods / James A. Lewis, John T. Fleming -- Mutagenesis / Philip Anderson -- Reverse genetics : from gene sequence to mutant worm / Ronald H.A. Plasterk -- Genetic mapping with polymorphic sequence-tagged sites / Benjamin D. Williams -- Genetic dissection of developmental pathways / Linda S. Huang, Paul W. Sternberg -- Mosaic analysis / Robert K. Herman -- Genetic balancers / Mark L. Edgley [and others] -- pt 2. Neurobiology. Genetic pharmacology : interactions between drugs and gene products in Caenorhabditis elegans / James B. Rand, Carl D. Johnson -- Methods of studying behavioral plasticity in Caenorhabditis elegans / Timothy N. Gannon, Catharine H. Rankin -- Laser killing of cells in Caenorhabditis elegans / Cornelia I. Bargmann, Leon Avery -- Electrophysiological methods / Leon Avery, David Raizen, Shawn Lockery -- pt. 3. Cell biology and molecular biology. Cell biology of nematode sperm / Steven W. L'Hernault, Thomas M. Roberts -- Blastomere culture and analysis / Lois G. Edgar -- Whole-mount in situ hybridization for the detection of RNA in Caenorhabditis elegans embryos / Geraldine Seydoux, Andrew Fire -- Fluorescence in situ hybridization for the detection of DNA and RNA / Donna G. Albertson, Rita M. Fishpool, Philip S. Birchall -- Immunofluorescence microscopy / David M. Miller, Diane C. Shakes -- Electron microscopy and three-dimensional image reconstruction / David H. Hall.
Proteins and protein assemblies / Henry F. Epstein, Feizhou Liu -- DNA transformation / Craig Mello, Andrew Fire -- Transcription and translation ; Techniques for analyzing transcription and translation / Michael Krause -- pt. 4. Genomics and informatics. The physical map of the Caenorhabditis elegans genome / Alan Coulson [and others] -- Genomic DNA sequencing methods / Anthony Favello, LaDeana Hillier, Richard K. Wilson -- Large-scale complementary DNA sequencing methods / Lucinda L. Fulton, LaDeana Hillier, Richard K. Wilson -- ACeDB and Macace / Frank H. Eeckman, Richard Durbin -- The Worm Community System, release 2.0 (WCSr2) / Laura M. Shoman [and others] -- Appendix A. Additional information and resources -- Appendix B. Caenorhabditis Genetics Center, laboratory designations -- Appendix C. Gene names and descriptions.
Abstract
:
The first of its kind, this laboratory handbook emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. Four primary sections--Genetic and Culture Methods, Neurobiology, Cell and Molecular Biology, and Genomics and Informatics--reflect the cross-disciplinary nature of C. elegans research. Because C. elegans is a simple and malleable organism with a small genome and few cell types, it provides an elegant demonstr.
Subject Term
:
Caenorhabditis elegans.
Caenorhabditis elegans -- genetics. (DNLM)D017173Q000235
Genetic Techniques. (DNLM)D005821
Cænorhabditis elegans.
SCIENCE -- Life Sciences -- Zoology -- General.
Caenorhabditis elegans. (OCoLC)fst00843844
Caenorhabditis elegans (DE-588)4147127-1
Molekularbiologie (DE-588)4039983-7
Cytologie (DE-588)4070177-3
Methode (DE-588)4038971-6
Genanalyse (DE-588)4200230-8
Genetik (DE-588)4071711-2
Neurobiologie (DE-588)4041871-6
Genom (DE-588)4156640-3
Celbiologie.
Genanalyse.
Genre
:
Electronic books.
Added Author
:
Epstein, Henry F., 1944-
Shakes, Diane C.,
Added Corporate Author
:
American Society for Cell Biology.
Electronic Access
:
| Shelf Number | Item Barcode | Shelf Location | Shelf Location | Holding Information |
|---|
| QH585 .M47 EB VOL. 48 | 1189450-1001 | Elsevier E-Book Collections | Elsevier E-Book Collections | |