Caenorhabditis elegans : modern biological analysis of an organism
by
 
Epstein, Henry F., 1944- editor.

Title
Caenorhabditis elegans : modern biological analysis of an organism

Author
Epstein, Henry F., 1944- editor.

ISBN
9780080859460
 
9780125641494

Physical Description
1 online resource (xxi, 659 pages) : illustrations

Series
Methods in cell biology, v. 48
 
Methods in cell biology ; v. 48.

General Note
"Prepared under the auspices of the American Society for Cell Biology."

Contents
pt. 1. Genetic and culture methods. Basic culture methods / James A. Lewis, John T. Fleming -- Mutagenesis / Philip Anderson -- Reverse genetics : from gene sequence to mutant worm / Ronald H.A. Plasterk -- Genetic mapping with polymorphic sequence-tagged sites / Benjamin D. Williams -- Genetic dissection of developmental pathways / Linda S. Huang, Paul W. Sternberg -- Mosaic analysis / Robert K. Herman -- Genetic balancers / Mark L. Edgley [and others] -- pt 2. Neurobiology. Genetic pharmacology : interactions between drugs and gene products in Caenorhabditis elegans / James B. Rand, Carl D. Johnson -- Methods of studying behavioral plasticity in Caenorhabditis elegans / Timothy N. Gannon, Catharine H. Rankin -- Laser killing of cells in Caenorhabditis elegans / Cornelia I. Bargmann, Leon Avery -- Electrophysiological methods / Leon Avery, David Raizen, Shawn Lockery -- pt. 3. Cell biology and molecular biology. Cell biology of nematode sperm / Steven W. L'Hernault, Thomas M. Roberts -- Blastomere culture and analysis / Lois G. Edgar -- Whole-mount in situ hybridization for the detection of RNA in Caenorhabditis elegans embryos / Geraldine Seydoux, Andrew Fire -- Fluorescence in situ hybridization for the detection of DNA and RNA / Donna G. Albertson, Rita M. Fishpool, Philip S. Birchall -- Immunofluorescence microscopy / David M. Miller, Diane C. Shakes -- Electron microscopy and three-dimensional image reconstruction / David H. Hall.
 
Proteins and protein assemblies / Henry F. Epstein, Feizhou Liu -- DNA transformation / Craig Mello, Andrew Fire -- Transcription and translation ; Techniques for analyzing transcription and translation / Michael Krause -- pt. 4. Genomics and informatics. The physical map of the Caenorhabditis elegans genome / Alan Coulson [and others] -- Genomic DNA sequencing methods / Anthony Favello, LaDeana Hillier, Richard K. Wilson -- Large-scale complementary DNA sequencing methods / Lucinda L. Fulton, LaDeana Hillier, Richard K. Wilson -- ACeDB and Macace / Frank H. Eeckman, Richard Durbin -- The Worm Community System, release 2.0 (WCSr2) / Laura M. Shoman [and others] -- Appendix A. Additional information and resources -- Appendix B. Caenorhabditis Genetics Center, laboratory designations -- Appendix C. Gene names and descriptions.

Abstract
The first of its kind, this laboratory handbook emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. Four primary sections--Genetic and Culture Methods, Neurobiology, Cell and Molecular Biology, and Genomics and Informatics--reflect the cross-disciplinary nature of C. elegans research. Because C. elegans is a simple and malleable organism with a small genome and few cell types, it provides an elegant demonstr.

Subject Term
Caenorhabditis elegans.
 
Caenorhabditis elegans -- genetics. (DNLM)D017173Q000235
 
Genetic Techniques. (DNLM)D005821
 
Cænorhabditis elegans.
 
SCIENCE -- Life Sciences -- Zoology -- General.
 
Caenorhabditis elegans. (OCoLC)fst00843844
 
Caenorhabditis elegans (DE-588)4147127-1
 
Molekularbiologie (DE-588)4039983-7
 
Cytologie (DE-588)4070177-3
 
Methode (DE-588)4038971-6
 
Genanalyse (DE-588)4200230-8
 
Genetik (DE-588)4071711-2
 
Neurobiologie (DE-588)4041871-6
 
Genom (DE-588)4156640-3
 
Celbiologie.
 
Genanalyse.

Genre
Electronic books.

Added Author
Epstein, Henry F., 1944-
 
Shakes, Diane C.,

Added Corporate Author
American Society for Cell Biology.

Electronic Access
ScienceDirect https://www.sciencedirect.com/science/book/9780125641494
 
ScienceDirect https://www.sciencedirect.com/science/bookseries/0091679X/48


Shelf NumberItem BarcodeShelf LocationShelf LocationHolding Information
QH585 .M47 EB VOL. 481189450-1001Elsevier E-Book CollectionsElsevier E-Book Collections