1. Expression Systems for Retroviral Proteases / 2. Genetic Approaches Designed to Minimize Cytotoxicity of Retroviral Protease / 3. Host Strain Selection for Bacterial Expression of Toxic Proteins / 4. Assay Methods for Retroviral Proteases / 5. Analysis of Human Immunodeficiency Virus Type 1 Protease Activity in Eukaryotic and Bacterial Cells / 6. Synthetic Approaches to Continuous Assays of Retroviral Proteases / 7. Identification, Purification, and Cell Culture Assays of Retroviral Proteases / 8. Stability of Dimeric Retroviral Proteases

9. Use of Steady State Kinetic Methods to Elucidate the Kinetic and Chemical Mechanisms of Retroviral Proteases / 10. X-Ray Structures of Retroviral Proteases and Their Inhibitor-Bound Complexes / 11. Probing Structure-Function Relationships in Human Immunodeficiency Virus Type 1 Protease via Molecular Dynamics Simulation / 12. Relationships of Human Immunodeficiency Virus Protease with Eukaryotic Aspartic Proteases / 13. Processing of Retroviral Gag Polyproteins: An in Vitro Approach / 14. Subsite Preferences of Retroviral Proteinases / 15. Specificity of Retroviral Proteases: An Analysis of Viral and Nonviral Protein Substrates

16. Rapid Determination of Endoprotease Specificity Using Peptide Mixtures and Edman Degradation Analysis / 17. Design of Tight-Binding Human Immunodeficiency Virus Type 1 Protease Inhibitors / 18. Design of Symmetry-Based, Peptidomimetic Inhibitors of Human Immunodeficiency Virus Protease / 19. Advances in Automated Docking Applied to Human Immunodeficiency Virus Type 1 Protease / 20. Use of Molecular Dynamics and Free Energy Perturbation Calculations in Anti-Human Immunodeficiency Virus Drug Design / 21. Qualitative Study of Drug Resistance in Retroviral Protease Using Structural Modeling and Site-Directed Mutagenesis