RNA methodologies : laboratory guide for isolation and characterization
by
Farrell, Robert E., Jr.
Title
:
RNA methodologies : laboratory guide for isolation and characterization
Author
:
Farrell, Robert E., Jr.
ISBN
:
9780123747273
9780080884950
9781282329980
9786612329982
Personal Author
:
Farrell, Robert E., Jr.
Edition
:
4th ed.
Publication Information
:
Amsterdam ; Boston : Elsevier/Academic Press, 2010.
Physical Description
:
1 online resource (xxiii, 717 pages) : illustrations
General Note
:
Previous edition: 2005.
Contents
:
CHAPTER 1: RNA and the Cellular Biochemistry Revisited -- CHAPTER 2: RNA Isolation Strategies -- CHAPTER 3: The Truth about Tissues -- CHAPTER 4: Going Green: RNA and the Molecular Biology of Plants (NEW) -- CHAPTER 5: Isolation of Polyadenylated RNA -- CHAPTER 6: Quality Control for RNA Preparations (EXPANDED) -- CHAPTER 7: Resilient Ribonucleases -- CHAPTER 8: Stringency: Conditions that Influence Nucleic Acid Structure (EXPANDED) -- CHAPTER 9: Electrophoresis of RNA (EXPANDED) -- CHAPTER 10: Photodocumentation and Image Analysis (EXPANDED) -- CHAPTER 11: Northern Analysis -- CHAPTER 12: Nucleic Acid Probe Technology -- CHAPTER 13: Practical Nucleic Acid Hybridization -- CHAPTER 14: Principles of Detection (EXPANDED) -- CHAPTER 15: Quantification of Specific mRNAs by Nuclease Protection -- CHAPTER 16: Analysis of Nuclear RNA (EXPANDED) -- CHAPTER 17: cDNA Synthesis (EXPANDED) -- CHAPTER 18: RT-PCR (NEW) -- CHAPTER 19: Quantitative PCR Techniques -- CHAPTER 20: Functional Genomics and Transcript Profiling (NEW) -- CHAPTER 21: High-Throughput Analysis of Gene Expression -- CHAPTER 22: Non-Array Methods for Global Analysis of Gene Expression (NEW) -- CHAPTER 23: RNA Interference: Targeted Gene Silencing (EXPANDED) -- CHAPTER 24: Genomes, Transcriptomes, Proteomes, and Bioinformatics -- CHAPTER 25: An RNA Paradigm.
Abstract
:
This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary.
Subject Term
:
RNA -- Analysis -- Laboratory manuals.
RNA -- isolation & purification. (DNLM)D012313Q000302
Molecular Biology -- methods. (DNLM)D008967Q000379
RNA -- physiology. (DNLM)D012313Q000502
SCIENCE -- Life Sciences -- Molecular Biology.
RNA -- Analysis. (OCoLC)fst01086245
Genre
:
Electronic books.
Laboratory Manual. (DNLM)D020484
Laboratory manuals. (OCoLC)fst01920776
Laboratory manuals.
Electronic Access
:
| Shelf Number | Item Barcode | Shelf Location | Shelf Location | Holding Information |
|---|
| QP623 .F37 2010 | 1193246-1001 | Elsevier E-Book Collections | Elsevier E-Book Collections | |