Urbanization and its impact on hydrology creates challenges for watershed management. In the last 25 years, Piedmont region of North Carolina shows approximately 28% growth in population. This study was done to assess the change in streamflow and baseflow characteristics in North Carolina streams in the Piedmont-Triad region to understand how urbanization has been influencing baseflow since the early to mid-20th Century. Long-term monitoring data were utilized to quantify baseflow and the annual water balance, and frequency analysis of Haw River at Haw River, Deep River at Ramseur, Yadkin River at Yadkin College, Rocky River near Norwood and North Buffalo Creek near Greensboro, and South Yadkin River near Mocksville watersheds (37.1 Square mile to 2,280 Square mile). The mean annual discharge coefficient (discharge/rainfall) of North Buffalo Creek and other watersheds from 1991 to 2015 was 0.46 and 0.31 (average of other five watersheds) respectively. The most urbanized watershed, North Buffalo Creek, had lower mean evapotranspiration rate (56%) than all other watersheds (63% to 72%). In the frequency analysis, stream flow shows the less time-lag in the discharge for most urbanized watershed than the all other watershed’s discharge. The Mann-Kendall trend analysis and regression analysis revealed decreasing baseflow trend with increasing urbanization only in Haw River, Yadkin River, and North Buffalo Creek watersheds. It was concluded in the study that North Buffalo Creek watershed had effect of urbanization on baseflow. But other watersheds did not show significant results in the baseflow variability due to urbanization. The results also proved that the baseflow analysis is very sensitive to the percent of impervious and pervious area available in the watersheds. It is also sensitive to the effect of the different factors like evapotranspiration, runoff accumulation, water leakage from the sources other than discharge in the stream, infiltration rate and topography of the watershed.