Optimization of PCRs / Michael A. Innis and David H. Gelfand -- Amplification of genomic DNA / Randall K. Saiki -- Amplification of RNA / Ernest S. Kawasaki -- RACE : rapid amplification of cDNA ends / Michael A. Frohman -- Degenerate primers for DNA amplification / Teresa Compton -- cDNA cloning using degenerate primers / Cheng Chi Lee and C. Thomas Caskey -- PCR with 7-Deaza-2'-deoxyguanosine triphosphate / Michael A. Innis -- Competitive PCR for quantitation of mRNA / Gary Gilliland, Steven Perrin, and H. Franklin Bunn -- Quantitative PCR / Alice M. Wang and David F. Mark -- Production of single-stranded DNA by asymmetric PCR / Peter C. McCabe -- Cloning with PCR / Stephen J. Scharf -- Oligonucleotide ligation assay / Ulf Landegren, Robert Kaiser, and Leroy Hood.

Nonisotopically labeled probes and primers / Corey Levenson and Chu-an Chang -- Incorporation of biotinylated dUTP / Y.-M. Dennis Lo, Wajahat Z. Mehal, and Kenneth A. Fleming -- Nonisotopic detection of PCR products / Rhea Helmuth -- Thermostable DNA polymerases / David H. Gelfand and Thomas J. White -- Procedures to minimize PCR-product carry-over / Shirley Kwok -- Sample preparation from blood, cells, and other fluids / Ernest S. Kawasaki -- Sample preparation from paraffin-embedded tissues / Deann K. Wright and M. Michele Manos -- Amplifying ancient DNA / Svante Pääbo -- In vitro transcription of PCR templates / Michael J. Holland and Michael A. Innis -- Recombinant PCR / Russell Higuchi -- DNase I footprinting / Barbara Krummel -- Sequencing with Taq DNA polymerase / Mary Ann D. Brow.

Direct sequencing with the aid of phage promoters / Steve S. Sommer [and others] -- Identifying DNA polymorphisms by denaturing gradient gel electrophoresis / Val C. Sheffield, David R. Cox, and Richard M. Meyers -- Amplification of flanking sequences by inverse PCR / Howard Ochman [and others] -- Detection of homologous recombinants / Michael A. Frohman and Gail R. Martin -- RNA processing : Apo-B / Lyn M. Powell -- A transcription-based amplification system / T.R. Gingeras [and others] -- Screening of [lambda] gt11 libraries / Kenneth D. Friedman [and others] -- HLA DNA typing / Henry A. Erlich and Teodorica L. Bugawan -- Multiplex PCR for the diagnosis of Duchenne muscular dystrophy / Jeffrey S. Chamberlain [and others] -- Isolation of DNA from fungal mycelia and single spores / Steven B. Lee and John W. Taylor -- Genetic prediction of hemophilia A / Scott C. Kogan and Jane Gitschier.

Haplotype analysis from single sperm or diploid cells / Ulf Gyllensten -- Amplification of ribosomal RNA genes for molecular evolution studies / Mitchell L. Sogin -- Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics / T.J. White [and others] -- Detection of human T-cell lymphoma/leukemia viruses / Garth D. Ehrlich, Steven Greenberg, and Mark A. Abbott -- Detection of Human Immunodeficiency Virus / David E. Kellogg and Shirley Kwok -- Detection of hepatitis B virus / I. Baginski [and others] -- Detection and typing of genital human papillomaviruses / Yi Ting and M. Michele Manos -- Detection of human cytomegalovirus / Darryl Shibata.

PCR amplification of enteroviruses / Harley A. Rotbart -- Novel viruses / David Mack, Oh-Sik Kwon, and Fred Faloona -- Analysis of Ras gene point mutations by PCR and oligonucleotide hybridization / John Lyons -- B-cell lymphoma : t(14;18) chromosome rearrangement / Marco Crescenzi -- Detecting bacterial pathogens in environmental water samples by using PCR and gene probes / Ronald M. Atlas and Asim K. Bej -- PCR in the diagnosis of retinoblastoma / Sang-Ho Park -- Determination of familial relationships / Cristián Orrego and Mary Claire King -- PCR in a teacup : a simple and inexpensive method for thermocycling PCRs / Robert Watson -- A low-cost air-driven cycling oven / Peter Denton and H. Reisner.

Modification of a Histokinette for use as an automated PCR machine / N.C.P. Cross [and others] -- Organizing a laboratory for PCR work / Cristián Orrego -- Basic equipment and supplies / Roberta Madej and Stephen Scharf.