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Thiol redox transitions in cell signaling. Part B, Cellular localization and signaling
Title:
Thiol redox transitions in cell signaling. Part B, Cellular localization and signaling
Author:
Cadenas, Enrique.
ISBN:
9780123810038
Edition:
1st ed.
Publication Information:
San Diego, Calif. : Elsevier, ©2010.
Physical Description:
1 online resource (xlviii, 340 pages) : illustrations.
Series:
Methods in enzymology, v. 474
Methods in enzymology ; v. 474.
Contents:
Engineering of Fluorescent Reporters into Redox Domains to Monitor Electron Transfers / Blot-Based Detection of Dehydroalanine-Containing Glutathione Peroxidase with the Use of Biotin-Conjugated Cysteamine / Analysis of the Redox Regulation of Protein Tyrosine Phosphatase Superfamily Members Utilizing a Cysteinyl-Labeling Assay / Measuring the Redox State of Cellular Peroxiredoxins by Immunoblottin / Thiol Redox Transitions by Thioredoxin and Thioredoxin-Binding Protein-2 in Cell Signaling / Detection of Protein Thiols in Mitochondrial Oxidative Phosphorylation Complexes and Associated Proteins / Mitochondrial Thioredoxin Reductase: Purification, Inhibitor Studies, and Role in Cell Signaling / Measuring Mitochondrial Protein Thiol Redox State / Measurement of Extracellular (Exofacial) Versus Intracellular Protein Thiols / Redox Clamp Model for Study of Extracellular Thiols and Disulfides in Redox Signaling / Redox State of Human Serum Albumin in Terms of Cysteine-34 in Health and Disease / Methods for Studying Redox Cycling of Thioredoxin in Mediating Preconditioning-Induced Survival Genes and Proteins / Oxidative Stress, Thiol Redox Signaling Methods in Epigenetics / Characterization of Protein Targets of Mammalian Thioredoxin Reductases / Alteration of Thioredoxin Reductase 1 Levels in Elucidating Cancer Etiology / Regulation of Apoptosis Signal-Regulating Kinase 1 in Redox Signaling / Protocols for the Detection of S-Glutathionylated and S-Nitrosylated Proteins In Situ / Synthesis, Quantification, Characterization, and Signaling Properties of Glutathionyl Conjugates of Enals / Thioredoxin and Redox Signaling in Vasculature--Studies Using Trx2 Endothelium-Specific Transgenic Mice
Abstract:
This volume, along with its companion (volume 475), presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to cell signaling. The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Over 450 volumes have been published to date, and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. *Along with companion volume, provides a full overview of techniques necessary to the study of thiol redox in relation to cell signaling * Gathers tried and tested techniques from global labs, offering both new and tried-and-true methods * Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines.
Genre:
Electronic Access:
ScienceDirect http://www.sciencedirect.com/science/book/9780123810038 ScienceDirect http://www.sciencedirect.com/science/bookseries/00766879/474 https://www.sciencedirect.com/science/bookseries/00766879/474Available:*
Shelf Number | Item Barcode | Shelf Location | Status |
|---|---|---|---|
| QP517 .C45 | 1179103-1001 | Elsevier E-Book Collections | Searching... |
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