Front Cover; International Review of Cytology, Volume 29; Copyright Page; Contents; List of Contributors; Chapter 1. Gram Staining and Its Molecular Mechanism; I. Introduction; II. Literature Review; III. A Proposed Mechanism for Gram Staining; IV. Conclusion; References; Chapter 2. The Surface Coats of Animal Cells; I . Introduction; II. Electron Microscope Histochemical Methods Used for the Demonstration of Surface Coats; III. Cell Coats; IV. Basal Laminas; V. External Laminas; VI. Conclusions; References; Supplemental References Added in Proof; Chapter 3. Carbohydrates in Cell Surfaces

I . IntroductionII. Architecture of the Plasma Membrane; III. Microscopic Demonstration of Cell Surface Carbohydrates; IV. Carbohydrate Content of Plasma Membranes; V. Nature of Carbohydrate-Containing Components of Plasma Membranes; VI. Modification of Cell Surfaces with Enzymes; VII. Cell Agglutinins; VIII. Surface Antigens; IX. Cell-Cell Recognition; X. Contact Inhibition and Malignant Transformation; XI. Biosynthesis of Carbohydrate-Containing Cell Membrane Components; XII. Some Conclusions and Speculations; References; Chapter 4. Differential Gene Activation in Isolated Chromosomes

I . IntroductionII. Isolated Interphase Chromosomes-The System; III. Structural Aspects of Interphase Chromosomes; IV. Chemical Composition of Interphase Chromosomes; V. The Role of Different Chromosomal Components in Gene Activation and Gene Activity; VI. Differential Gene Activation in Isolated Chromosomes-The Experiment; VII. Biological Significance of the Ionic Effects on Chromosomal Template Activity; VIII. Conclusions and Extrapolation; References; Note added in proof; Chapter 5. Intraribosomal Environment of the Nascent Peptide Chain; I. Introduction; II. Ribosomes

III. Steps in Protein SynthesisIV. Summary; References; Chapter 6. Location and Measurement of Enzymes in Single Cells by Isotopic Methods; Part I. Principles and Light Microscope Applications; I. Enzymes and the Single Cell; II. Principles of the Labeled Inhibitor Method; III. The Application of Radioautography; IV. Applications to Specific Enzymes; V. Validation of Radioautographic Measurements by Other Techniques; VI. Advantages and Limitations of the Labeled Inhibitor Approach; References; Chapter 7. Location and Measurement of Enzymes in Single Cells by Isotopic Methods

Part II. Application to Electron MicroscopyI. Introduction; II. Electron Microscope Radioautography; III. Application to Specific Enzymes; References; Chapter 8. Neuronal and Glial Perikarya Preparations: An Appraisal of Present Methods; I. Introduction; II. The Preparation of Neuronal and/or Glial Suspensions; III. Isolation of Cell Types from Suspensions; IV. Assessment of Purity of Fractions; V. The Question of Cellular Integrity; VI. The Future of Research on Isolated Neuronal and Glial Perikarya; References; Chapter 9. Functional Electron Microscopy of the Hypothalamic Median Eminence