Cover; Contents; List of Contributors; Preface; Part A: Isolation of Nuclei and Preparation of Chromatin. I; Chapter 1. Methods for Isolation of Nuclei and Nucleoli; I. Isolation of Nuclei; II. The Sucrose-Calcium Procedure for Isolation of Nuclei; Ill. The Citric Acid Procedure for Isolation of Nuclei; IV. The Use of Detergents for Nuclear Isolation; V. Isolation and Purification of Nucleoli; VI. The Sonication Procedure for Isolation of Nucleoli; VII. Compression-Decompression Procedure for Isolation of Nucleoli; VIII. ""Nucleolar"" Preparations Obtained by Chemical Extractions; References

Chapter 2. Nonaqueous Isolation of Nuclei from Cultured CellsI. Introduction; II. Lyophilization Apparatus; III. Cell Culture; IV. Preparation of Cells for Freezing; V. Freezing Cells; VI . Drying Cells; VII. Homogenization; VIII. Separation of Cell Fractions; IX. Methods of Handling Glycerol Suspensions; X. Appearance of Purified Nuclei; XI. Recovery of Nuclear DNA; XII. Distributions of RNA Species; XIII. Electrophoresis of Nuclear and Cytoplasmic Proteins; XIV. ATP Content of Whole Cells and of Isolated Nuclei; XV. Conclusions; References

Chapter 3. Isolation of Nuclei Using Hexylene GlycolI. Introduction; II. General Methods; III. Specific Nuclear Isolation Methods; IV. Nuclear Characterization; V. Discussion; References; Chapter 4. Isolation of Nuclei and Preparation of Chromatin from Plant Tissues; I. Introduction; II. Isolation of Nuclei and Chromatin from Maize Tissues; III. Concluding Remarks; IV. List of Buffers; Note Added in Proof; References; Chapter 5. Methods for the Isolation of Nuclei from Ciliated Protozoans; I. Introduction; II. Isolation Procedures; III. Other Criteria for Selection of Method

IV. Conclusions and PerspectivesReferences; Chapter 6 . The Isolation of Nuclei from Fungi; I. Introduction; II. Isolation of Nuclei from Physarum polycephalum; III. Isolation of Nuclei from Neurospora crussu; IV. Conclusion; References; Part B: Nuclear-Cytoplasmic Exchange; Chapter 7. Methods for Nuclear Transplantation in Amphibia; I. Introduction; II. The Preparation of Nuclei for Injection; III. Enucleation; IV. The Transplantation Procedure; V. Culture; VI. Labeling; VII. Applications of Nuclear Transplantation Techniques in Amphibia to the Study of Chromosomal Proteins

Note Added in ProofReferences; Chapter 8. Methods for Studying Nucleocytoplasmic Exchange of Nonhistone Proteins in Embryos; I. Introduction; II. Materials; III. Procedures for Obtaining Embryos and Oocytes; IV. Radioactive Labeling of Embryos and Oocytes; V. Nucleocytoplasmic Exchange of Nonhistones; VI. Cytological Procedures; VII. Concluding Remarks; References; Chapter 9. Methods for Studying Nucleocytoplasmic Exchange of Macromolecules; I. Introduction; II. Methods; III. Results; IV. Conclusions and Applications; References; Part C: Fractionation and Characterization of Histones. I