Front Cover; Motility Assays for Motor Proteins; Copyright Page; Contents; Contributors; Preface; Chapter 1. In Vitro Methods for Measuring Force and Velocity of the Actin-Myosin Interaction Using Purified Proteins; I. Introduction; II. Preparation of in Vitro Motility Assay Components; III. In Vitro Assay for Myosin Velocity; IV. In Vitro Assay for Myosin Force in the Motility Assay; V. Components of the Optical Trap System; VI. Future Directions; References; Chapter 2. Myosin-Specific Adaptations of the Motility Assay; I. Introduction; II. Description of Equipment

III. Basic Assay ProcedureIV. Adaptations of the Assay; V. Quantitation of Rate of Actin Filament Sliding; References; Chapter 3. Motility of Myosin I on Planar Lipid Surfaces; I. Introduction; II. Methods; III. Evaluation of the Method; References; Chapter 4. Microtubule and Axoneme Gliding Assays for Force Production by Microtubule Motor Proteins; I. Introduction; II. Microtubule Gliding Assay; III. Polarity of Force Production; References; Chapter 5. Analyzing Microtubule Motors in Real Time; I. Introduction; II. Configuration of the Computer-Video Microscope Setup

III. The Microtubule Motility AssayIV. Overview of Typical Results Obtained with the Real-Time Motility Assay; V. Conclusions; Addendum; References; Chapter 6. Assays of Axonemal Dynein-Driven Motility; I. Introduction; II. Dark-Field Video Microscopy; III. Procedures and Analysis of ATP-Induced Microtubule Sliding in Flagellar Axonemes; IV. The Second Assay: Dynein-Driven Microtubule Translocation Using Purified Proteins; V. Summary: Future; References; Chapter 7. Preparation of Marked Microtubules for the Assay of the Polarity of Microtubule-Based Motors by Fluorescence Microscopy

I. IntroductionII. Preparation of Labeled Microtubules; III. Reagents; IV. Preparation of the Bright Microtubule Seeds; V. Preparation of the Polarity-Marked Microtubules; VI. Visualization of Marked Microtubules in Motor Polarity Assays; References; Chapter 8. Expression of Microtubule Motor Proteins in Bacteria for Characterization in in Vitro Motility Assays; I. Introduction; II. Construction of Plasmids for Expression in Bacteria; III. Vectors Used for Expression of Motor Proteins; IV. Expression of Proteins in Escherichia coli

V. Partial Purification of Proteins from Bacteria for in Vitro Motility AssaysVI. Brief Description of Motility Assays for Testing the Functional Properties of the Expressed Proteins; VII. Microtubule Binding Assays; VIII. Measurement of ATPase Activity as a Determinant of Motor Protein Function; IX. Problems Encountered with Prokaryotic Expression Systems and Some Solutions; References; Chapter 9. Tracking Nanometer Movements of Single Motor Molecules; I. Introduction; II. Movements by Single Motor Molecules; III. Microtubule Movements on Motor-Coated Glass; IV. Conclusion; References