Front Cover; Methods in Cell Biology, Volume 23; Copyright Page; Contents; List of Contributors; Preface; Chapter 1. Introduction; Text; References; Part I: Protein Synthesis and Posttranslational Modifications; Chapter 2. Synthesis and Assembly of Transmembrane Viral and Cellular Glycoproteins; I. Introduction; II. Transmembrane Biosynthesis of the VSV G Protein; III. Interaction of Glycoprotein mRNA and Microsomal Membranes; IV. Biogenesis of Two Sindbis Virus Glycoproteins; V. Interaction of Nascent Sindbis Glycoprotein and the Endoplasmic Reticulum

VI. Movement of Transmembrane Viral Glycoproteins from the Rough Endoplasmic Reticulum to the Plasma MembraneVII. Processing of the Asparagine-Linked Oligosaccharide Chains of VSV; VIII. Mutants in G Defective in Maturation; References; Chapter 3. The Genetics of Protein Secretion in Escherichia coli; I. Introduction; II. Genetics of Protein Secretion; III. Gene Fusions in the Study of Secretion; IV. Deleterious Effects of Hybrid Protein Synthesis on Bacterial Growth; V. Isolation of MBP and ?rec Signal Sequence Mutants; VI. Amino Acid Changes in Signal Sequence Mutants; VII. Conclusion

II. Insulin BiosynthesisIII. Glucagon Biosynthesis; References; Chapter 7. Synthesis and Processing of Asparagine-Linked Oligosaccharides of Glycoproteins; I. Introduction; II. Oligosaccharide-Lipid; III. Protein Glycosylation; IV. Glycoprotein Processing; References; Chapter 8. Glycosylation Steps Involved in Processing of Pro-Corticotropin-Endorphin in Mouse Pituitary Tumor Cells; I. Introduction; II. Approaches Used to Study Proteolytic Processing and Glycosylation of the Precursor in Mouse Pituitary Tumor Cells; III. Intermediate and End Products of Processing of Pro-ACTH-Endorphin

IV. Details of Glycosylation Steps Involved in Processing of Pro-ACTH-EndorphinV. Use of the Glycosylation Inhibitor, Tunicamycin, to Determine If Cleavage Sites Are Specified by Oligosaccharides; VI. Structure of the ACTH-Endorphin Peptides from Tunicamycin-Treated Cells; References; Chapter 9. Posttranslational Events in Collagen Biosynthesis; I. Introduction; II. Genetic Types of Collagen; III. Posttranslational Modifications; IV. Role of Posttranslational Modifications in the Secretory Process; V. Disorders of Collagen Secretion; References