Front Cover; The Enzymes, Volume XIX; Copyright Page; Contents; Preface; Chapter 1. Binding Energy and Catalysis; I. Introduction; II. Relationship of Binding to Catalysis in Unimolecular Reactions; III. Beyond Unimolecular Reactions: Entropy; IV. Indirect Uses of Binding; V. Prospects; References; Chapter 2. Biological Electron Transfer; Introduction; I. Thermodynamics of Electron Transfer Reactions; II. Kinetics of Electron Transfer Reactions; III. Microscopic Theory of Electron Transfer; IV. Examples of Electron Transfer Reactions; V. Future Prospects; References

Chapter 3. Steady-State KineticsI. Introduction; II. Nomenclature and Notation; III. BasicTheory; IV. Derivation of Rate Equations; V. Determination of Kinetic Mechanism; VI. Stickiness and Methods for Measurement; VII. Determination of Chemical Mechanism; VIII. Concluding Remarks; References; Chapter 4. Analysis of Protein Function by Mutagenesis; Introduction; I. Structural Aspects of Mutant Enzymes; II. Substrate Binding and Catalysis; III. Engineering New Specificities; IV. Conclusions; References; Chapter 5. Mechanism-Based (Suicide) Enzyme Inactivation; I. Introduction

II. Dehydrases and IsomerasesIII. Pyridoxal Phosphate-Dependent Enzymes; IV. Flavin-Dependent Enzymes; V. Hemoprotein Enzymes; VI. Nonheme Metallonzymes; VII. Quinoproteins; VIII. Hydrolytic Enzymes; IX. Enzymes of Nucleotide Modification; X. Summary; References; Chapter 6. Site-Specific Modification of Enzyme Sites; I. Introduction; II. General Characteristics of Site-Specific Modification; III. Haloketones; IV. Aldehyde Affinity Labels; V. Fluorosulfonylbenzoyl Nucleosides; VI. Photoreactive Compounds; VII. Haloacyl and Alkyl Halide Derivatives; VIII. Miscellaneous Affinity Labels

IX. ConclusionsReferences; Chapter 7. Stereochemistry of Enzyme-Catalyzed Reactions at Carbon; I. Introduction; II . Nicotinamide-Dependent Dehydrogenases: Hydride Transfer to Carbon; III. Flavin-Dependent Oxidoreductases: Hydride Transfer to Nitrogen; IV. lsomerases Involving Proton Transfer: Importance of Single-Base Mechanisms; V. Enzyme-Catalyzed Aldol- and Claisen-Type Condensations: ß-Keto Acid Decarboxylases: Stereochemistry versus Stability of Reaction Intermediates; VI. Pyridoxal Phosphate- and Pyruvyl-Dependent Enzymes: Flexible Response to Different Chemical Challenges

VII. ß-Elimination-Addition Reactions: E2 versus E1cB MechanismsVIII. Methyl Group Transfer Reactions: One- versus Two-step Mechanisms; IX. Concluding Remarks; References; Author Index; Subject lndex