The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * Thermodynamics as a tool for understanding molecular logic * Thermal denaturation methods in the study of protein folding * Predicting thermodynamic properties of RNA * Sedimentation equilibrium as a thermodynamic tool * Molecular volume * Thermodynamic parameters from hydrogen exchange measurements.