Cover -- front cover -- copyright -- Table of Contents -- Contributors to Volume 392 -- Methods in Enzymology -- [1] Transgene-Induced RNA Interference as a Tool for Plant Functional Genomics -- [2] Constructs and Methods for Hairpin RNA-Mediated Gene Silencing in Plants -- [3] RNA Interference in Caenorhabditis elegans -- [4] High-Throughput RNA Interference Screens in Drosophila Tissue Culture Cells -- [5] Mechanistic Insights Aid Computational Short Interfering RNA Design -- [6] Novel Methods for Expressing RNA Interference in Human Cells -- [7] Delivery of Small Interfering RNA to Mammalian Cells in Culture by Using Cationic Lipid/Polymer-Based Transfection Reagents -- [8] Subcellular Distribution of Small Interfering RNA: Directed Delivery Through RNA Polymerase III Expression Cassettes and Localization by In Situ Hybridization -- [9] Viral Delivery of Recombinant Short Hairpin RNAs -- [10] Targeting Cellular Genes with PCR Cassettes Expressing Short Interfering RNAs -- [11] Use of Short Hairpin RNA Expression Vectors to Study Mammalian Neural Development -- [12] Analysis of T-Cell Development by Using Short Interfering RNA to Knock Down Protein Expression -- [13] Lentiviral Vector Delivery of Recombinant Small Interfering RNA Expression Cassettes -- [14] Paradigms for Conditional Expression of RNA Interference Molecules for Use Against Viral Targets -- [15] High-Throughput RNA Interference Strategies for Target Discovery and Validation by Using Synthetic Short Interfering RNAs: Functional Genomics Investigations of Biological Pathways -- [16] RNA Interference In Vivo: Toward Synthetic Small Inhibitory RNA-Based Therapeutics -- [17] Labeling and Characterization of Small RNAs Associated with the RNA Interference Effector Complex RITS -- [18] RNA Interference Spreading in C. elegans -- [19] Human Dicer: Purification, Properties, and Interaction with PAZ PIWI Domain Proteins -- [20] Delivery of siRNA and siRNA Expression Constructs to Adult Mammals by Hydrodynamic Intravascular Injection -- [21] Analysis of Short Interfering RNA Function in RNA Interference by Using Drosophila Embryo Extracts and Schneider Cells -- [22] Use of RNA Polymerase II to Transcribe Artificial MicroRNAs -- [23] Adeno-Associated Virus Vectors for Short Hairpin RNA Expression -- [24] Simple, Robust Strategies for Generating DNA-Directed RNA Interference Constructs -- Author Index -- Subject Index -- Last Page.