Front Cover -- Separation Methods in Drug Synthesis and Purification -- Copyright Page -- Contents -- Editor's Preface -- Series Editor's Preface -- List of Contributors -- Chapter 1. Comparison of various modes and phase systems for analytical HPLC -- 1.1 Fundamentals of HPLC -- 1.2 Chromatographic column and column packing particles -- 1.3 Separation modes in HPLC -- 1.4 Method development and optimisation of conditions in isocratic HPLC -- 1.5 Development of gradient-elution separations -- 1.6 Acknowledgements -- 1.7 References -- Chapter 2. Fast generic HPLC methods -- 2.1 Introduction.

2.2 Theory -- 2.3 Strategy for production of fast gradients -- 2.4 Fast gradients in practice -- 2.5 References -- Chapter 3. Application of standard methods in capillary electrophoresis for drug analysis -- 3.1 Introduction to capillary electrophoresis -- 3.2 Analysis of pharmaceuticals by CE -- 3.3 Low-pH buffer for analysis of basic drugs -- 3.4 High-pH buffer for analysis of acidic drugs -- 3.5 Micellar electrokinetic chromatography (MEKC) for neutral and/or charged drugs -- 3.6 Microemulsion electrokinetic chromatography (MEEKC) for neutral and/or charged drugs.

3.7 Indirect UV detection method for analysis of inorganic anions -- 3.8 Indirect UV detection method for analysis of simple organic acids -- 3.9 Indirect UV detection method for analysis of metal ions -- 3.10 Non-aqueous CE for analysis of acidic and basic drugs -- 3.11 Benefits of adopting standard CE methods -- 3.12 References -- Chapter 4. Capillary electrochromatography (CEC) -- 4.1 Introduction -- 4.2 Basic principles of capillary electrochromatography -- 4.3 Mobile phase composition -- 4.4 Stationary phases used in CEC -- 4.5 Operational characteristics of CEC.

4.6 Gradient and pressure-assisted (pseudo) CEC -- 4.7 Conclusions -- 4.8 Glossary of symbols -- 4.9 References -- Chapter 5. Coupled chromatography-mass spectrometry techniques for the analysis of combinatorial libraries -- 5.1 Introduction -- 5.2 LC/MS analysis of high-throughput parallel synthesis libraries -- 5.3 Example for monitoring the rehearsal phase of the synthesis of a solid-phase library -- 5.4 LC/UV/MS as a pre-screen for autoprep-solution phase -- 5.5 Assisted automated LC/MS analysis -- 5.6 The analysis of split-pool combinatorial libraries -- 5.7 Conclusions and future.

5.8 References -- Chapter 6. Optimization strategies for HPLC and CZE -- 6.1 Introduction -- 6.2 Responses and response functions -- 6.3 Univariate optimization strategies -- 6.4 Factorial methods -- 6.5 Mixture designs -- 6.6 Robustness/ruggedness -- 6.7 The simplex sequential approach -- 6.8 Automating the whole process: expert systems and knowledge based systems -- 6.9 References -- Chapter 7. Strategies for the development of process chromatography as a unit operation for the pharmaceutical industry -- 7.1 Introduction -- 7.2 The process development cycle.