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G protein pathways. Part C, Effector mechanisms
Title:
G protein pathways. Part C, Effector mechanisms
Author:
Iyengar, Ravi.
ISBN:
9780080496931
9780121822460
Publication Information:
San Diego : Academic Press, ©2002.
Physical Description:
1 online resource (1 volume) : illustrations (some color).
Series:
Methods in enzymology ; v. 345
Methods in enzymology ; v. 345.
Contents:
Front Cover; G Protein Pathways; Copyright Page; Table of Contents; Contributors to Volume 345; Preface; Volume in Series; Section I: Modeling Intracellular Signaling Pathways; Chapter 1. Use of Kinetikit and GENESIS for Modeling Signaling Pathways; Section II: Phosphodiesterases; Chapter 2. Assays of G Protein/cGMP-Phosphodiesterase Interactions; Chapter 3. Assaying G Protein-Phosphodiesterase Interactions in Sensory Systems; Section III: Calcium and Potassium Channels; Chapter 4. Studies of Endogenous G-Protein-Mediated Pathways in Neurons by Whole-Cell Electrophysiology
Chapter 5. Biochemical Approaches to Study Interaction of Calcium Channels with RGS12 in Primary Neuronal CulturesChapter 6. Assaying Phosphatidylinositol Bisphosphate Regulation of Potassium Channels; Section IV: Adenylyl Cyclases; Chapter 7. Purification of Soluble Adenylyl Cyclase; Chapter 8. Calcium-Sensitive Adenylyl Cyclase/Aequorin Chimeras as Sensitive Probes for Discrete Modes of Elevation of Cytosolic Calcium; Chapter 9. Kinetic Analysis of the Action of P-Site Analogs
Chapter 17. Construction of Soluble Adenylyl Cyclase from Human Membrane-Bound Type 7 Adenylyl CyclaseChapter 18. Genetic Selection of Regulatory Mutants of Mammalian Adenylyl Cyclases; Section V: Phospholipases and Lipid-Derived Products; Chapter 19. Expression and Characterization of Rat Brain Phospholipase D; Chapter 20. G-Protein-Coupled Receptor Regulation of Phospholipase D; Chapter 21. Analysis and Quantitation of Ceramide; Chapter 22. Assays for Phospholipase D Reaction Products
Chapter 23. Determination of Strength and Specificity of Membrane-Bound G Protein-Phospholipase C Association Using Fluorescence SpectroscopyChapter 24. Assays and Characterization of Mammalian Phosphatidylinositol 4,5-Bisphosphate-Sensitive Phospholipase D; Chapter 25. Characterization and Purification of Phosphatidylinositol Trisphosphate 5-Phosphatase from Rat Brain Tissues; Section VI: Small GTP-Binding Proteins; Chapter 26. Assay of Cdc42, Rac, and Rho GTPase Activation by Affinity Methods; Chapter 27. Assays of ADP-Ribosylation Factor Function
Abstract:
This third volume in the trio covering G proteins, features integrated approaches to studying G proteins. Methods pertaining to signaling mechanisms are presented, including theoretical and modeling approaches, biochemistry and molecular biology, and cell biology and physiology. The techniques for studying the structure and function of G proteins are important not only to those with specific research interests in them, but also endocrinologists and pharmacologists conducting research on signaling mechanisms that are increasingly understood to interact with G proteins.
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Electronic Access:
ScienceDirect http://www.sciencedirect.com/science/book/9780121822460 ScienceDirect http://www.sciencedirect.com/science/bookseries/00766879/345 https://www.sciencedirect.com/science/bookseries/00766879/345Available:*
Shelf Number | Item Barcode | Shelf Location | Status |
|---|---|---|---|
| QP601 .M4 EB PT. C | 1188535-1001 | Elsevier E-Book Collections | Searching... |
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