Why choose mammalian cells for protein production? / Savvas C. Makrides, Holly L. Prentice -- Vectors for gene expression in mammalian cells / Savvas C. Makrides -- Virus-based vectors for gene expression in mammalian cells. Herpes simplex virus / Edward A Burton, Qing Bai, William F. Goins, David J. Fink, Joseph C. Glorioso -- Virus-based vectors for gene expression in mammalian cells. Epstein-Barr virus / Gregory Kennedy, Bill Sugden -- Virus-based vectors for gene expression in mammalian cells. SV40 / David S. Strayer, Pierre Cordelier, Julien Landré, Alexei Matskevitch, Hayley J. McKee, Carmen N. Nichols, Martyn K. White, Marlene S. Strayer -- Virus-based vectors for gene expression in mammalian cells. Adeno-associated virus / Xiao Xiao -- Virus-based vectors for gene expression in mammalian cells. Adenovirus / Denis Bourbeau, Yué Zeng, Bernard Massie -- Virus-based vectors for gene expression in mammalian cells. Vaccinia virus / Miles W. Carroll, Gerald R. Kovacs -- Virus-based vectors for gene expression in mammalian cells. Baculovirus / J. Patrick Condreay, Thomas A. Kost -- Virus-based vectors for gene expression in mammalian cells. Coronavirus / Luis Enjuanes, Fernando Almazán, Javier Ortego -- Virus-based vectors for gene expression in mammalian cells. Poliovirus / Shane Crotty, Raul Andino -- Virus-based vectors for gene expression in mammalian cells. Sindbis virus / Henry V. Huang, Sondra Schlesinger -- Virus-based vectors for gene expression in mammalian cells. Semliki Forest virus / Kenneth Lundstrom -- Virus-based vectors for gene expression in mammalian cells. Retrovirus / Cristina Parolin, Giorgio Palù -- Virus-based vectors for gene expression in mammalian cells. Lentiviruses / Mehdi Gasmi, Flossie Wong-Staal -- Methods for DNA introduction into mammalian cells / Pamela A. Norton, Catherine J. Pachuk -- Lipid reagents for DNA transfer into mammalian cells / Christophe Masson, Virginie Escriou, Michel Bessodes, Daniel Scherman -- Reporter genes for monitoring gene expression in mammalian cells / Jawed Alam, Julia L Cook -- Gene transfer and gene amplification in mammalian cells / Florian M. Wurm, Martin Jordan -- Co-transfer of multiple plasmids/viruses as an attractive method to introduce several genes in mammalian cells / Martin Jordan, Florian M. Wurm -- Optimization of plasmid backbone for gene expression in mammalian cells / Pascal Bigey, Marie Carrière, Daniel Scherman -- Use of scaffold/matrix-attachment regions for protein production / Pierre-Alain Girod, Nicolas Mermod -- Chromatin insulators and position effects / David W. Emery, Mari Aker, George Stamatoyannopoulos -- Locus control regions / Xiangdong Fang, Kenneth R. Peterson, Qiliang Li, George Stamatoyannopoulos -- Protein synthesis, folding, modification, and secretion in mammalian cells / M. Stacey Arnold, Randal J. Kaufman -- Pathways and functions of mammalian protein glycosylation / Dale A. Cumming -- Metabolic engineering of mammalian cells for higher protein yield / Hitto Kaufmann, Martin Fussenegger -- Translational regulation in mammalian cells / Marilyn Kozak -- Pathways of mammalian messenger RNA degradation / Angela Inácio, Stephen A. Liebhaber -- Pathways of mammalian protein degradation / William A. Dunn Jr. -- Stabilization of proteasomal substrates by viral repeats / Nico P. Dantuma, Maria G. Masucci -- Architecture and utilization of highly expressed genomic sites / Jürgen Bode, Sandra Götze, Ellen Ernst, Yves Hüsemann, Alexandra Baer, Jost Seibler, Christian Mielke -- Intracellular targeting of antibodies in mammalian cells / Quan Zhu, Wayne A. Marasco -- Inducible gene expression in mammalian cells / Wilfried Weber, Martin Fussenegger -- Protein production by large-scale mammalian cell culture / Liangzhi Xie, Weichang Zhou, David Robinson -- Protein production in transgenic animals / Yann Echelard, Harry M. Meade -- Strategies for the purification of recombinant proteins / Steven L. Giardina.