Cellular and molecular procedures in developmental biology
Title:
Cellular and molecular procedures in developmental biology
Author:
De Pablo, Flora, editor.
ISBN:
9780080584591
9780121531362
9781281712967
Publication Information:
San Diego : Academic Press, ©1998.
Physical Description:
1 online resource (xvi, 329 pages) : illustrations.
Series:
Current topics in developmental biology ; v. 36
Current topics in developmental biology ; v. 36.
Contents:
Avian embryo as a model in developmental studies : chimeras and in vitro clonal analysis / Elisabeth Dupin, Catherine Ziller, and Nicole M. Le Douarin -- Inhibition of gene expression by antisense oligonucleotides in chick embryos in vitro and in vivo / Aixa V. Morales and Flora de Pablo -- Lineage analysis using retroviral vectors / Constance L. Cepko [and others] -- Use of dominant negative constructs to modulate gene expression / Giorgio Lagna and Ali Hemmati-Brivanlou -- Use of embryonic stem cells for the genetic manipulation of the mouse / Miguel Torres -- Organoculture of otic vesicle and ganglion / Juan J. Garrido [and others] -- Organcoculture of the chick embryonic neuroretina / Enrique J. de la Rosa, Begona Diaz, and Flora de Pablo -- Embryonic explant and slice preparations for studies of cell migration and axon guidance / Catherine E. Krull and Paul M. Kulesa -- Culture of avian sympathetic neurons / Alexander V. Holst and Hermann Roher -- Analysis of gene expression in cultured primary neurons / Ming-Ji Fann and Paul H. Patterson -- Selective aggregation assays for embryonic brain cells and cell lines / Shinichi Nakagawa, Hiroaki Matsunami, and Masatoshi Takeichi -- Flow cytometric analysis of whole organs and embryos / Jose Serna, Belen Pimentel, and Enrique J. de la Rosa -- Detection of multiple gene products simulaneously by in situ hybridization and immunohistochemistry in whole mounts of avian embryos / Claudio D. Stern -- Cloning of genes from single neurons / Catherine Dulac -- Methods for detecting and quantifying apoptosis / Nicola J. McCarthy and Gerard I. Evan -- Methods in drosophila cell cycle biology / Fabian Feiguin, Salud Llamazares, and Cayetano Gonzalez -- Single central nervous system neurons in culture / Juan Lerma, Miquel Morales, and Maria de los Angeles Vicente -- Patch-clamp recordings from drosophila presynaptic terminals / Manuel Martinez-Padron and Alberto Ferrus.
Abstract:
This volume incorporates practical methods at the level of molecular, cellular, and whole organism biology in vertebrate and invertebrate models. It presents straightforward protocols written step-by-step for state-of-the-art techniques with the emphasis on single-cell resolution procedures. Key Features * Provides straightforward, current protocols and critical appraisals * Includes diverse analysis of cellular and molecular techniques * Presents everything from whole-organ cultures to electrophysiological approaches * Details a variety of methods for interfering with gene function in various species * Offers multiple illusions of in situ hybridization, immunostaining, and apoptosis.
Subject Term:
Genre:
Electronic Access:
ScienceDirect http://www.sciencedirect.com/science/book/9780121531362 ScienceDirect http://www.sciencedirect.com/science/bookseries/00702153/36Available:*
Shelf Number | Item Barcode | Shelf Location | Status |
|---|---|---|---|
| QL955 .C87 EB VOL. 36 | 1189762-1001 | Elsevier E-Book Collections | Searching... |
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