Aqueous two-phase systems
Title:
Aqueous two-phase systems
Author:
Walter, Harry, editor.
ISBN:
9780121821296
Publication Information:
San Diego : Academic Press, ©1994.
Physical Description:
1 online resource (xxx, 725 pages) : illustrations.
Series:
Methods in enzymology, v. 228
Methods in enzymology ; v. 228.
Contents:
Section I. General methodology and apparatus -- Section II. Partitioning and affinity partitioning of macromolecules -- A. Proteins -- B. Nucleic acids -- Section III. Studies on protein interactions, molecular structure, charge, hydrophobicity, and conformational changes -- Section IV. Partitioning and affinity partitioning of particulates -- A. Cells: Separation and fractionation of cell populations and obtaining information on their surface properties -- Affinity partitioning -- B. Organelles: Separation and subfractionation -- C. Membranes: Separation and subfractionation -- Affinity partitioning -- Membrane domain analysis -- Section V. Aqueous phase separations in biological systems -- Section VI. Aqueous two-phase systems in large-scale processes: Biotechnology -- A. Extractive bioconversions -- B. Proteins: Downstream processing -- C. Design of proteins for enhanced extraction -- D. Other applications of aqueous phases in biotechnology.
Abstract:
This volume of Methods in Enzymology represents the sole collection of separation methods based on aqueous two-phase systems. It includes procedures for the isolation of proteins, especially enzymes, nucleic acids, cell membranes, and organelles, as well as for the separation and study of cells. Key Features * Use of affinity partitioning for enzyme purification * Separation and study of cells * Isolation of plasma membranes * Large scale biotechnical use.
Subject Term:
Genre:
Electronic Access:
ScienceDirect http://www.sciencedirect.com/science/book/9780121821296 ScienceDirect http://www.sciencedirect.com/science/bookseries/00766879/228 ScienceDirect https://www.sciencedirect.com/science/bookseries/00766879/228Available:*
Shelf Number | Item Barcode | Shelf Location | Status |
|---|---|---|---|
| QP601 .M49 VOL. 228 | 1191876-1001 | Elsevier E-Book Collections | Searching... |
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