1. Analysis of gene function using DNA microarrays / 2. An introduction to microarray data analysis and visualization / 3. Genome-wide approaches to monitor pre-mRNA splicing / 4. ChIP-Seq: Using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites / 5. Genome-wide mapping of nucleosomes in yeast / 6. Genome-wide translational profiling by ribosome footprinting / 7. Synthetic genetic array (SGA) analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe / 8. Making temperature-sensitive mutants / 9. Quantitative genetic interaction mapping using the E-MAP approach / 10. Exploring gene function and drug action using chemogenomic dosage assays / 11. Yeast expression proteomics by high-resolution mass spectrometry / 12. High-quality binary interactome mapping / 13. Quantitative analysis of protein phosphorylation on a system-wide scale by mass spectrometry-based proteomics / 14. A toolkit of protein-fragment complementation assays for studying and dissecting large-scale and dynamic protein-protein interactions in living cells / 15. Yeast lipid analysis and quantification by mass spectrometry / 16. Mass spectrometry-based metabolomics of yeast / 17. Imaging single mRNA molecules in yeast / 18. Reconstructing gene histories in Ascomycota fungi / 19. Experimental evolution in yeast: a practical guide / 20. Enhancing stress resistance and production phenotypes through transcriptome engineering / 21. Visualizing yeast chromosomes and nuclear architecture / 22. Quantitative localization of chromosomal loci by immunofluorescence / 23. Spinning disk confocal microscopy of yeast / 24. Correlative GFP-immunoelectron microscopy in yeast / 25. Analyzing P-bodies and stress granules in Saccharomyces cerevisiae / 26. Analyzing mRNA expression using single mRNA resolution fluorescent in situ hybridization / 27. The Use of in vitro assays to measure endoplasmic reticulum-associated degradation (ERAD) / 28. A protein transformation protocol for introducing yeast prion particles into yeast / 29. Overexpression and purification of integral membrane proteins in yeast / 30. Biochemical, cell biological and genetic assays to analyze amyloid and prion aggregation in yeast / 31. Genetics and molecular biology in Candida albicans / 32. Molecular genetics of Schizosaccharomyces pombe / 33. Applying genetics and molecular biology to the study of the human pathogen Cryptococcus neoformans / 34. The Fungal Genome Initiative and lessons learned from genome sequencing / 35. Ultradian metabolic cycles in yeast