The importance of cellular organization cannot be overstated in the proper functioning and structure of biological tissues and organs, and it is thus, it is vital to be able to control this organization in order to succeed in the creation of replacements and repairs for damaged and diseased organs and tissues. The goal of this research was to investigate several different methods for effecting desired cellular organization through the environment, including the use of both mechanical forces and placement of cells into favorable environments for growth and function. A method for endothelial cell seeding via magnetic forces was demonstrated that both delivers HUVEC cells into an ECM-like scaffold more effectively than gravitational seeding alone, and allows for the ability to achieve a single-cell dispersion for seeding. A second study attempts to utilize bone marrow mononuclear cells to improve angiogenesis and so oxygenation and oxygen-sensing around implanted sensors in the face of the foreign body reaction, while also examining the effects of implants on local and systemic gene expression for a wide variety of genetic markers over time, finding a potential depletion of circulating stem-like cells in the blood in response to implantation, as well as a maintenance of stem-like markers at the implant site when implants are co-delivered with bone marrow mononuclear cells. Last, a study examined the effects of both cyclic strain amplitude and cyclic strain frequency on the F-actin fiber alignment of endothelial (HUVEC) cells, finding an increasingly perpendicular (to the direction of stretch) fiber orientation with increasing strain amplitudes, as well as a difference in fiber orientation despite similar strain rates (the product of amplitude and frequency) when different amplitudes and frequencies were used in combination.