The Southern California Bight receives approximately 1.5 million gallons of wastewater effluent per day. Although regional monitoring has occurred periodically since the implementation of wastewater treatment practices, few studies have attempted to investigate long-term fish population or environmental changes in response to changes in wastewater treatment. The present study compared Pacific sanddab, California lizardfish, and English sole populations and environmental parameters near a wastewater outfall site and far-field site located in Orange County, California, between 1990 and 2015. Biannual fish trawl data and environmental data (surface temperature, bottom temperature, salinity, dissolved oxygen) collected by Orange County Sanitation District's Ocean Monitoring Program were analyzed using a two-way analysis of variance and regression analysis. Overall, there was no interaction between sample sites and treatment periods on the fish population (except English sole abundance) and environmental parameters analyzed. Fish abundance and relative condition factor did not differ significantly between sites, but all fish species were significantly larger at the outfall site. Wastewater effluent did not appear to adversely impact the three fish populations or the four environmental parameters investigated in this study. Further monitoring and investigation into other indicators of pollution are necessary to manage and further asses the effects of wastewater discharge.