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Methods in cell biology. Volume 40, A practical guide to the study of calcium in living cells
Title:
Methods in cell biology. Volume 40, A practical guide to the study of calcium in living cells
Author:
Nuccitelli, Richard.
ISBN:
9780125641418
9781281514097
9786611514099
9780125228107
9780080859385
Publication Information:
San Diego : Academic Press, ©1994.
Physical Description:
1 online resource (xiii, 368 pages) : illustrations.
Series:
Methods in cell biology ; v. 40
Methods in cell biology ; v. 40.
Contents:
Front Cover; Methods in Cell Biology: A Practical Guide to the Study of Calcium in Living Cells; Copyright Page; Contents; Contributors; Preface; Part I: Ca2+ Buffers and [Ca2+]i Perturbation Techniques; Chapter 1. A Practical Guide to the Preparation of Ca2+ Buffers; I. Introduction; II. Which Ca2+ Buffer Should You Use?; III. Basic Mathematical Relationships; IV. Temperature, Ionic Strength, and pH Corrections; V. Ca2+ Measurement and Calibration Solutions; VI. Preparing the Buffer Solution; VII. MaxChelator (MAXC v6.50): An Easy-to-Use Computer Program for Ca2+ Buffer Preparation
V. Potential Applications of Intracellular Ca2+ Pump Inhibitors in Calcium Cell BiologyReferences; Part II: Microelectrode Techniques for Measuring [Ca2+]i and Ca2+ Fluxes; Chapter 4. How to Make and to Use Calcium-Specific Mini- and Microelectrodes; I. Introduction; II. General Considerations; III. Minielectrodes; IV. Microelectrodes; V. Troubleshooting; References; Chapter 5. The Vibrating Ca2+ Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca2+ Influx and Efflux; I. Introduction; II. Principles of Vibrating Electrodes; III. Construction of Ion-Selective Probes
IV. Calibration and SensitivityV. Data Acquisition; VI. Data Analysis and Examples; References; Chapter 6. Application of Patch Clamp Methods to the Study of Calcium Currents and Calcium Channels; I. Introduction; II. Pipettes and Solutions; III. Patch Configurations; IV. Reconstitution of Purified Channel Proteins; V. Expression Cloning of Channel Subunits; VI. Pancreatic ß Cell: A Model System for Analysis of Ca2+ Signaling; References; Part III: Fluorescence Techniques for Imaging [Ca2+]i; Chapter 7. Practical Aspects of Measuring [Ca2+] with Fluorescent Indicators; I. Introduction
II. Fluorescent Ca2+ IndicatorsIII. Loading Indicators into Cells; IV. Manipulation of [Ca2+]; V. Conversion of Indicator Fluorescence Signal into Values of [Ca2+]i; VI. Concluding Remarks; References; Chapter 8. Rapid Simultaneous Estimation of Intracellular Calcium and pH; I. Introduction; II. Simultaneous lntracellular Calcium and pH Measurements; III. Correction Procedures and Calculations; IV. Choosing Calcium and pH Indicator Dyes; V. Possible (but untried) Dye Combinations; VI. Equipment; VII. Results; References
Abstract:
A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. Key Features * Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes * Fluorescent dyes * Luminescent proteins * Includes techniques for perturbing intracellular Ca2+ * Covers detailed methodology plus problems and pitfalls of each technique * Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program * Color plates illustrate techniques such as * Confocal ratio-imaging * Use of aequorin.
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Electronic Access:
ScienceDirect http://www.sciencedirect.com/science/book/9780125641418Available:*
Shelf Number | Item Barcode | Shelf Location | Status |
|---|---|---|---|
| QH585 .M4840 1994 EB | 1179426-1001 | Elsevier E-Book Collections | Searching... |
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