Front Cover; The Enzymes: Mechanisms of Catalysis; Copyright Page; Contents; Preface; Chapter 1. Transient-State Kinetic Analysis of Enzyme Reaction Pathways; I. Introduction; II. Rationale for Transient Kinetic Analysis; III. Experimental Methods for Complete Kinetic Analysis; IV. Kinetics of Substrate Binding and Catalysis; V. Measurement of Reaction Rates at Enzyme Active Sites; VI. Methods of Data Fitting; VII. Résumé; References; Chapter 2. Metal Ions at Enzyme Active Sites; I. General Considerations; II. Properties of Metal Ion-Ligand Complexes

III. Methods to Study Metal Ion Environments in ProteinsIV. Metal Ion-Assisted Phosphoryl Transfer Reactions; References; Chapter 3. Phosphate Ester Hydrolysis; I. Introduction; II. Methods for Syntheses and Configurational Analyses of Oxygen Chiral Phosphate and Thiophosphate Esters; III. Nonenzymic Phosphate Ester Hydrolysis; IV. Enzymic Phosphate Ester Hydrolysis; References; Chapter 4. Nucleotidyltransferases and Phosphotransferases: Stereochemistry and Covalent Intermediates; I. Introduction; II. Stereochemistry of Phospho and Nucleotidyl Transfer; III. Nucleotidyltransferases

IV. PhosphotransferasesV. ATP-Dependent Synthetases and Ligases; VI. Importance of Covalent Intermediates; VII. Transition State for Phospho Transfer; References; Chapter 5. Glycosidases and Glycosyltransferases; I. Introduction; II. Lysozyme; III. Glycosidase Reversible Inhibitors; IV. Glycosidase Catalytic Flexibility: Catalysis of Substrates with Very Small Aglycon; V. ß-Galactosidase; VI. Sucrose Glycosyltransferases; VII. Glycosidase and Glycosyltransferase Structure; References; Chapter 6. Catalytic Strategies in Enzymic Carboxylation and Decarboxylation; I. Introduction

II. Some Background ChemistryIII. lnterconversion of Pyruvate and Oxaloacetate; IV. ß-Keto Acid Decarboxylations Not Involving a Metal Ion; V. Ribulose-1,5-bisphosphate Carboxylase; VI. Schiff Base-Dependent Decarboxylations; VII. Thiamin Pyrophosphate-Dependent Reactions; VIII. Conclusion; References; Chapter 7. Mechanisms of Enzymic Carbon-Carbon Bond Formation and Cleavage; I. Introduction; II. Enzymes in Carbon-Carbon Bond Formation; III. Mechanistic Patterns in Carbon-Carbon Bond Formation without Coenzymes

IV. Mechanistic Patterns for Carbon-Carbon Bond Formation Involving Selected CoenzymesV. Concluding Remarks; References; Chapter 8. Enzymic Free Radical Mechanisms; I. Ribonucleotide Reductases; II. Monoamine Oxidase; III. DNA Photolyase; IV. Pyruvate Formate-Lyase; V. Other Radical Enzymes; References; Chapter 9. Molecular Mechanism of Oxygen Activation by P-450; I. Introduction; II. Structure and Reactivity of P-450; III. Mechanism of Oxygen Activation by P-450; IV. Summary; References; Chapter 10. Mechanism of NAD-Dependent Enzymes; I. Mechanism of NAD-Dependent Dehydrogenases