Cover image; Title page; Table of Contents; Copyright; Foreword; Preface; Acknowledgments; Section I: Protein Microsequence Methods; Chapter 1: Protein and Peptide Recovery from PVDF Membranes; Publisher Summary; I Introduction; II Materials and Methods; III Results and Discussion; IV Conclusions; Acknowledgements; Chapter 2: Automated C-Terminal Sequencing of Peptides; Publisher Summary; I Introduction; II EXPERIMENTAL; III RESULTS AND DISCUSSION; Acknowledgments; Chapter 3: Elution and Internal Amino Acid Sequencing of PVDF-Blotted Proteins; Publisher Summary; I Introduction; II Methods.

III ResultsIV Discussion; Chapter 4: Evaluation of Protein Sequencing Core Facilities: Design, Characterization, and Results from a Test Sample (ABRF-91SEQ); Publisher Summary; I Introduction; II Materials and Methods; III Results and Discussion; Acknowledgements; Chapter 5: OPTIMIZATION OF SOLID PHASE PEPTIDE SEQUENCING; Publisher Summary; I INTRODUCTION; II MATERIALS AND METHODS; III RESULTS; IV DISCUSSION; Chapter 6: Evaluation of the Blott Cartridge for Enhanced Gas Phase Sequencing at Maximum Sensitivity; Publisher Summary; I Introduction; II Materials and Methods.

III Results and DiscussionIV Summary of Blott Cartridge Runs; V Reaction Program Modifications; VI Conclusions; Acknowledgements; Chapter 7: Reusing PVDF Electroblotted Protein Samples After N-Terminal Sequencing To Obtain Unique Internal Amino Acid Sequence; Publisher Summary; I Introduction; II Materials and Methods; III Results and Discussion; IV Conclusions; Acknowledgements; Chapter 8: CHROMATOGRAPHIC CARBON AS AN INERT SAMPLE ADSORBENT FOR PROTEIN SEQUENCING; Publisher Summary; I INTRODUCTION; II Experimental; III Results; V DISCUSSION.

Chapter 9: N[alpha]-ACYLAMINOACYL-PEPTIDE HYDROLASE: SPECIFICITY AND USE TO UNBLOCK N-ACETYLATED PROTEINSPublisher Summary; I INTRODUCTION; II EXPERIMENTAL; III RESULTS AND DISCUSSION; IV SUMMARY AND CONCLUSIONS; ACKNOWLEDGEMENTS; Section II: Protein Microsequencing Workshop; Chapter 10: Strategies for the Isolation of Peptides from Low-Abundance Proteins for Internal Sequence Analysis; Publisher Summary; I Introduction; II Experimental Section; III Results and Discussion; IV Conclusions; Acknowledgements.

Chapter 11: High-Sensitivity Peptide Mapping Utilizing Reversed-Phase Microbore and Microcolumn Liquid ChromatographyPublisher Summary; I Introduction; II Experimental; III Results and Discussion; Chapter 12: MICROSCALE PROTEIN ISOLATION AND MICROSEQUENCING WORKSHOP: AN OVERVIEW; Publisher Summary; I INTRODUCTION; II ANALYSIS OF PURIFIED PROTEINS; III PEPTIDE MAPPING; Section III: Peptide/Protein Separation Techniques; Chapter 13: Micropreparative Separation of Tryptic Digests by Capillary Electrophoresis and Characterization by Protein Sequencing; Publisher Summary; Introduction.