Part 1: Introduction to Fluorescence Microscopy: -- J.S. Ploem, Fluorescence Microscopy. -- Part II: Optical Probes and Their Applications: -- F.H. Kasten, Introduction to Fluorescent Probes: Properties, History, and Applications. -- R.P. Haugland and I.D. Johnson, Intracellular Ion Indicators. V.L. Singer and R.P. Haugland, Fluorescent Imaging of Nucleic Acids and Protein in Gels. -- Part III: Using Optical Probes in Cells -- Practicalities, Problems, and Pitfalls: -- D.N. Bowser, S.H. Cody, P.N. Dubbin, and D.A. Williams, Introducing and Calibrating Fluorescent Probes in Cells and Organelles. -- F. Bobanovic, Electroporation: A Method for Introduction of Non-permeable Molecular Probes. -- S.L. Shorte and S. Bolsover, Imaging Reality: Understanding Maps of Physiological Cell Signals Measured by Fluorescence Microscopy and Digital Imaging. -- J. Hoyland, Fluorescent Probes in Practice -- Potential Artifacts. -- Part IV: Optical Probes for Specific Molecules, Organelles, and Cells: -- A.V. Zelenin, Acridine Orange as a Probe for Molecular and Cell Biology. -- J.W. Kok and D. Hoekstra, Fluorescent Lipid Analogues: Applications in Cell and Membrane Biology. -- S.R. Adams, B.J. Bacskai, S.S. Taylor and R.Y. Tsien, Optical Probes for Cyclic AMP. -- Part V: Technology for Qulaitative and Quantitative Detection of Optical Probes in Living Cells: -- W.T. Mason, J. Dempster, J. Hoyland, T.J. McCann, B. Somasundaram and W. O'Brien, Quantitative Digital Imaging of Biological Activity in Living Cells with Ion-sensitive Fluorescent Probes. -- J. Dempster, Fast Photomeric Measurements of Cell Function. -- L.M. Loew, Potentiometric Membrane Dyes and Imaging Membrane Potential in Single Cells. -- J.-y. Wu, L.B. Cohen, and C.X. Falk, Fast Multisite Optical Measurement of Membrane Potential, with Two Examples. -- S. Antic and D. Zecevic, Imagine Membrane Potential Changes in Individual Neurons. -- Part VI: Using Novel Indications for Genetic, Molecular, and Cellular Function: -- G.B. Sala-Newby, J.M. Kendall, Helen E. Jones, Kathryn M. Taylor, Michael N. Badminton, David H. Llewellyn and A.K. Campbell, Bioluminescent and Chemiluminescent Indicators for Molecular Signaling and Function in Living Cells. -- G.A. Rutter, A.A. Culbert and J.M. Tavare, Luminescence Imaging of Gene Expression in Single Living Cells. -- S.R. Kain, Enhanced Variants of the Green Fluorescent Protein for Greater Sensitivity, Different Colors, and Detection of Apoptosis. -- R. Haggart, N. Bodsworth, and N. Foote, Rapid Detection of Microorganisms. -- Part VII: Applications of Confocal Microscopy for Optical Probe Imaging: -- C.J.R. Sheppard, Confocal Microscopy -- Principles, Practice, and Options. -- K.-P. Yip and I. Kurtz, Dual-excitation Confocal Fluorescence Microscopy. -- Y.S. Prakash, M.S. Kannan, and G.C. Sieck, High-speed Confocal Imaging in Four Dimensions. -- G. Cox and C. Sheppard, Multiphoton Fluorescence Microscopy. -- P. Lipp and M.D. Bootman, High-resolution Confocal Imaging of Elementary Ca2+ Signals in Living Cells. -- A. Ichihara, T. Tanaami, H. Ishida, and M. Shimizu, Confocal Fluorescent Microscopy Using Nipkow Scanner. -- S.H. Cody and D.A. Williams, Optimizing Confical Microscopy for Thick Biological Specimens. -- B.R. Masters and B. Chance, Redox Confocal Imaging: Intrinsic Fluorescent Probes of Cellular Metabolism. -- Part VIII: Advanced Imaging and Light Detection Approaches for Optical Probe Applications: -- G.J. Puppels, Confocal Raman Microspectroscopy. -- C. Rothmann, I. Bar-Am, Z. Dubinsky, S. Katz, and Z. Malik, Spectral Imaging of Autofluorescence Molecules and DNA Probes. -- B. Masters, P.T.C. So, and E. Gratton, Multiphoton Excitation Microscopy and Spectroscopy of Cells, Tissues, and Human Skin In Vivo. -- R. Wolthuis, T.C. Bakker Schut, P.J. Caspers, H.P.J. Buschman, T.J. R~mer, H.A. Bruining, and G.J. Puppels, Raman Spectroscopic Methods for In Vitro and In Vivo Tissue Characterization. -- G.J. Puppels, J.M.C.C. Coremans, and H.A. Bruining, In Vivo Semiquantitative NADH-fluorescence Imaging. -- T.W.J. Gadella Jr., Fluorescence Lifetime Imaging Microscopy (FLIM): Instrumentation and Applications. -- G. Skews and T. McCann, Detection of Fluorescently Labelled Proteins following Gel Electrophoresis: A Key Enabling Technology for Proteomics. -- Part IX: CCD Cameras: Key Enabling Technologies for Optical Probe Imaging: -- P. Tomkins and A. Lyons, Properties of Low-light-level Intensified Cameras. -- R. Aikens, Properties of Low-light-level Slow-scan Detectors. -- C.D. Mackay, High-speed Digital CCD Cameras -- Principles and Applications. -- Part X: Flow Cytomeric Methodologies for Measurement of Optical Probes in Living Cells: -- E.R. Simons, Flow Cytometry: Use of Multiparameter Kinetics to Evaluate Several Activation Parameters Simultaneously in Individual Living Cells. -- A.M. Gurney, Photolabile Caged Compounds. -- K. Hahn, J. Kolega, J. Montibeller, R. DeBasio, P. Post, J. Myers and D.L. Taylor, Fluorescent Analogues: Optical Biosensors of the Chemical and Molecular Dynamics of Macromolecules in Living Cells. -- Part XI: Applications of Optical Probe Imaging to Biological Problems: -- M. Fricker, C. Plieth, H. Knight, E. Blancaflor, M.R. Knight, N.S. White, S. Gilroy, Fluorescence and Luminescence Techniques to Probe Ion Activities in Living Plant Cells. -- G. Shamkar and M.A. Horton, Nuclear Calcium: Concepts and Controversies. -- A.W. de Feijter, J.E. Trosko, and M.H. Wade, Assessment of Gap Junctional Intercellular Communications in Living Cells Using Fluorescence Techniques. -- K.E. Sawin, J.A. Theriot and T.J. Mitchison, Photoactivation of Fluorescence as a Probe for Cytoskeletal Dynamics in Mitosis and Cell Motility. -- K. Florine-Casteel, J.L. Lemasters and B. Herman, Video Imaging of Lipid Order. -- Index.