Sect. 1. Energetic coupling in enzymatic reactions -- Energetics of substate binding, catalysis, and product release -- Energy coupling through molecular discrimination: nicotinate phosphoribosyltransferase -- On the advantage of imperfect energetic linkage -- Reaction dynamics of G-protein catalyzed hydrolysis of GFP as viewed by x-ray crystallographic snapshots of Gi[alpha]₁ -- Energetics of nucleotide hydrolysis in polymer assembly/disassembly: the cases of actin and tubulin -- Fundamental mechansisms of substrate channeling -- Sect. 2. Intermediates and complexes in catalysis -- Intermediates and energetics in pyruvate phosphate dikinase -- Raman spectroscopic studies of the structures, energetics, and bond disortions of substrates bound to enzymes -- Crystallographic analysis of solvent-trapped intermediates to chymotrypsin -- Sect. 3. Detection and properties of low-barrier hydrogen bonds -- Nuclear magnetic resonance methods for the detection and study of low-barrier hydrogen bonds of enzymes -- Hydrogen bonding in enzymatic catalysis: analysis of energetic contributions -- Application of marcus rate theory to proton transfer in enzyme-catalyzed reactions -- Sect. 4. Transition state determination and inhibitors -- Enzymatic transition-state analysis and transition-state analogues -- Determining transition states from kinetic isotope effects -- Computational methods fro transition state and inhibitor recognition.