Preface -- Acknowledgement -- Contributors.- Specimen Collection, Handling and Processing.- DNA/RNA Isolation and Quantitation -- Test Validation -- In Vitro Amplification Methods in Molecular Diagnostics.-Whole Genome Sequencing in the Clinical Laboratory.-Estimation of Exon Dosage Using Real-Time Quantitative Polymerase Chain Reaction -- Reporting Clinical Molecular Genetic Laboratory Results -- HIV-1 Genotyping and Virtual Phenotyping -- COBAS® Ampliprep/Taqman HIV-1 Test -- Hepatitis C Virus (HCV) Genotype Assay (LiPA) -- COBAS® Ampliprep/Taqman HCV Test -- Epstein-Barr Virus and Cytomegalovirus Viral Load Monitoring by Quantitative Real-Time Polymerase Chain Reaction.-Quantification of BK Viral Load.-Molecular Diagnosis and Epidemiology of Rabies -- Pyrosequencing for Bacterial Identification.-Other Pathogens of Significant Public Health Concern -- Gene Rearrangements Testing.-Mutation Analysis in Cancer.-Minimal Residual Disease -- Pyrosequencing in Cancer -- Clinical Applications of Microarrays in Cancer.-Basic Theories and Clinical Applications of Molecular Flow Cytometry.-Molecular Prenatal Diagnostic Testing -- PCR Based Diagnosis of Fragile X Syndrome -- Microsatellite Testing Short Tandem Repeat Profile Generation -- Parentage.-Diagnostic Molecular Training -- Molecular Diagnostics Point of Care Testing Training.