Tool use is an integral part of human society. There are few, if any, aspects of human civilization that do not utilize tools in some form. The advent of computers has led to the development of virtual tools which include the computer mouse and surgical robot. The use of tools has been studied both behaviorally and functionally; however virtual tools have received significantly less attention. Studies that have focused on reaction time associated with tool mechanics knowledge, which would include understanding the connection between the handle (i.e. the portion of the tool controlled by the user) and effector (i.e. the portion of the tool that interacts with the environment). Past research has shown that loss of this information results in an increased reaction time cost that can be attenuated when knowledge of the tool's mechanics are present or recalled. Using an MRI compatible virtual tool paradigm, the chapters of this dissertation will address numerous aspects of virtual tool use. The paradigm utilized in the subsequent chapters is a touchpad controlled object on a monitor that has a handle, effector, and connection between the two that was used to hit targets on the screen. By limiting view of the connector or handle as well as introduction of reversal conditions (i.e. conditions in which the user's movements are reversed from the movement of the effector due to a pivot point), numerous experimental conditions were created. The first aim was to adapt a preexisting virtual tool paradigm into a touch pad dependent paradigm that could be safely used in an MRI. The second aim was to use this novel paradigm during fMRI scanning sessions to determine the neurological correlates associated tool mechanics. The third aim was to address the neurological areas associated with use of a complex tool. The fourth and final aims were to examine the brain regions supporting the recall of learned virtual tools. Results showed that the MRI compatible virtual tool paradigm was behaviorally matched to previous research utilizing a virtual tool. Furthermore, behavioral results showed the expected reaction time cost associated with the loss of tool mechanics knowledge as well as the attenuation of that cost during memory recall. The experiments in the following chapters show activity within the areas of the brain that process motor planning, motor execution, and memory. Specifically, these experiments support roles for the lateral occipital complex (LOC), posterior middle temporal gyrus (pMTG), supramarginal gyrus (SMG), precuneus, superior parietal lobule (SPL), dorsal premotor cortex (PMd), and supplemental motor cortex (SMA) in the use of tools. The activity of the LOC was shown to have a specific role in processing reversal conditions, suggesting an expansion of its role in tool semantic knowledge. The pMTG was thought to be involved in processing the connection between the handle and the effector. The superior SMG and anterior intraparietal sulcus (aIPS) is related to a fast track process that decreases the reaction time of movement. Finally, the precuneus was may serve as a processing center that integrates existing actions and predict future actions.