Purpose: To assess North Carolina dental hygiene (DH), nursing students' and practitioners' knowledge, opinions, and behaviors regarding e-cigarettes. Methods: A convenience sample of DH students, registered dental hygienists (RDHs), nursing students and registered nurses (RNs) completed surveys to assess practice behaviors, knowledge, opinions, and curriculum/training about E-cigarettes. Data was analyzed with Cochran-Mantel-Haenszel tests. Results: Students (n = 146) and 144 practitioners completed surveys. Only 55.0% of DH and 18.5% nursing students reported E-cigarettes were included in their curriculum. DH students were more likely than nursing students to always ask about cigarette and e-cigarette use in health histories, to advise patients to quit e-cigarettes, and to know that e-cigarettes deliver less nicotine per puff and hold similar health risks to smokeless tobacco. RNs were more likely to refer patients for quitting assistance than RDHs. Conclusion: DH and nursing curricula and continuing education programs must address e-cigarettes, their health effects, and how to deliver tobacco/alternative tobacco product cessation counseling.