Front Cover; Nuclear Structure and Function; Copyright Page; Contents; Contributors; Preface; Part I: Cell Fractionation Preparation and Characterization of Probes; Chapter 1. Isolation and Characterization of Karyoskeletal Protein-Enriched Fractions from Vertebrate Livers; Chapter 2. Preparation of Karyoskeletal Protein-Enriched Fractions from Drosophila melanogaster Cells and Tissues; Chapter 3. Isolation of Nuclei and Nucleoli from the Yeast Saccharomyces cerevisiae; Part II: Structural Analysis of the Interphase Nucleus

Chapter 4. Determining Nuclear Structure Using the Fluorescence Light MicroscopeChapter 5. Localization of Single Nuclear Pore Complexes by Confocal Laser Scanning Microscopy and Analysis of Their Distribution; Chapter 6. Nuclear Ultrastructure: Transmission Electron Microscopy and Image Analysis; Chapter 7. Three-Dimensional Surface Structure Analysis of the Nucleus; Chapter 8. Scanning Transmission Electron Microscopy of Nuclear Structures; Part III: Chromatin and Subnuclear Structures; Chapter 9. Electron Microscopic Imaging of Chromatin with Nucleosome Resolution

Chapter 10. Mapping Three-Dimensional Chromosome Architecture in SituChapter 11. Structural Analysis of Meiotic Chromosomes and Synaptonemal Complexes in Higher Vertebrates; Chapter 12. Genetic and Morphological Approaches for the Analysis of Meiotic Chromosomes in Yeast; Chapter 13. Mapping Proteins to Nuclear Pore Complexes by Immunogold Electron Microscopy; Chapter 14. Methods Used to Study Structure and Function of the Nucleolus; Chapter 15. Identification of Base-Unpairing Region-Binding Proteins and Characterization of Their in Vivo Binding Sequences

Part IV: Nuclear Assembly/Disassembly in Cell-Free SystemsChapter 16. Cell-Free Nuclear Reassembly in Mammalian Mitotic Homogenates; Chapter 17. Analysis of Nuclear Envelope Assembly Using Extracts of Xenopus Eggs; Chapter 18. Cell-Free Nuclear Assembly and Disassembly in Drosophila; Chapter 19. Methods for Studying in Vitro Assembly of Male Pronuclei Using Oocyte Extracts from Marine Invertebrates: Sea Urchins and Surf Clams; Part V: Localization of Nuclear Processes; Chapter 20. Mapping of DNA Replication Sites in Situ by Fluorescence Microscopy

Chapter 21. EM Visualization of Transcriptionally Active Genes after Injection into Xenopus Oocyte NucleiChapter 22. Cell-Free Systems to Study Chromatin Remodeling; Chapter 23. In Vitro Systems for the Reconstitution of snRNP and Protein Nuclear Import; Chapter 24. In Vivo Nuclear Transport Kinetics in Saccharomyces cerevisiae; Chapter 25. Nuclear Transport of RNAs in Microinjected Xenopus Oocytes; Chapter 26. In Vivo Systems to Study the Dynamics of Nuclear Lamins; Index