Microenvironmental Control of Epigenetic Transitions during Human Somatic Cell Reprogramming
Başlık:
Microenvironmental Control of Epigenetic Transitions during Human Somatic Cell Reprogramming
Yazar:
Harkness, Ty, author.
ISBN:
9780438001107
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (231 pages)
Genel Not:
Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B.
Advisors: Krishanu Saha Committee members: Aseem Z. Ansari; Randolph S. Ashton; William L. Murphy; Sridharan Rupa.
Özet:
Somatic cell reprogramming enables the generation of self-renewing and patient-specific cell sources for drug discovery, cell therapies, and tissue engineering. The ability to faithfully and efficiently reprogram cells to uniform target populations could have far-reaching consequences in biomedical research and patient care. Reprogramming cell cultures, however, are notoriously multivariate, asynchronous, and inefficient. Thus far, studies undertaken to improve reprogramming methods have largely relied on onerous optimization and guesswork focused on genetic manipulation or addition of soluble factors to overcome epigenetic barriers. These challenges motivate the mechanistic study of reprogramming processes under conditions allowing for precise and independent control of signaling inputs. This thesis aimed to combine rational engineering of the reprogramming microenvironment with innovative materials science, biological, and engineering techniques to collect large, high quality, reproducible data sets that could be analyzed and understood through multivariate computational modeling. Using this basic strategy we were able to generate testable hypotheses to understand and engineer epigenetic transitions. The resulting studies represent tangible steps toward a mechanistic understanding of human somatic cell reprogramming, which is critical to the rapid clinical adaption of reprogramming technologies.
Notlar:
School code: 0262
Tüzel Kişi Ek Girişi:
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(677854.1) | 677854-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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