Boston University Henry M. Goldman School of Dental Medicine
Başlık:
Boston University Henry M. Goldman School of Dental Medicine
Yazar:
Alwafi, Hanadi A., author.
ISBN:
9780438041981
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (230 pages)
Genel Not:
Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B.
Advisors: Laisheng Chou Committee members: Dan Nathanson; Athanasios Zavras.
Özet:
Background: Thymoquinone (TQ) is one of the most active ingredients of Nigella sativa seed. TQ has a variety of pharmacologic properties including possess antinociceptive, antibacterial, anti-inflammatory, anti-oxidative activities and osteogenic effects on bone cells. Because of these properties, TQ might play an important role in odontogenic effects on human dental pulp cells and be used as a pulp capping material.
Purpose: This study evaluates the effect of TQ on the attachment efficiency, proliferation, and odontogenic differentiation of Human Dental Pulp Cells (HDPC's). The effect of TQ on odontogenic differentiation was also determined by evaluating alkaline phosphatase (ALP) activity and Dentin Sialoprotein (DSP) expression.
Methods: Human dental pulp cells were cultured in triplicate using growth media with various TQ concentrations: 5 muM, 10muM, 15muM, 30muM, and 10muM as a control group at 7 and 21 days. Crystal violet staining was used to determine cell attachment efficiency and cell proliferation. The proliferation rates were normalized to cell numbers of each group at 16 hours. Cell differentiation was assessed by evaluating ALP activity and DSP expression. The data were normalized on per million cells basis. Univariate analyses including ANOVA and Student's t-test were conducted.
Results: Higher cell attachment efficiency was shown in all TQ groups at 16 hours (P<. 0001) except for the 5 muM group. A significantly higher cell proliferation rate was shown with low TQ concentration 5 muM at 7 days (P<. 0001) and at 21days (P<. 0.05). However, the cell proliferation rates decreased significantly with higher TQ concentrations at both time intervals ( P< 0.0001). Similarly, prolifration rates decreased at 21 days TQ =10 muM (P< 0.0001) and TQ =15 muM (P=0.0006). Significantly higher levels of alkaline phosphatase activity were observed in all TQ groups at 7 days (P <0.0001) and at 21 days (P <0.0001). Dentin sialoprotein expression was significantly down regulated in all TQ groups at 7 days compared to the control ( P <0.05), however, at day 21 only TQ =15 muM exhibited significant down regulation of DSP compared to the control (P <0.0001).
Conclusion: All tested TQ concentrations significantly enhanced cell attachment efficiency at 16 hours except TQ =5 muM. Cell proliferation rates were increased significantly by low TQ concentration 5 muM, and decreased significantly with higher TQ concentration 30 muM at 7 and 21 days. TQ exhibits odontogenic potential by inducing a significant increase in ALP activity at 7 and 21 days. TQ did not have any effect on DSP expression at 21 days except TQ =15 muM which signficantly decreased DSP expression.
Notlar:
School code: 0017
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(682849.1) | 682849-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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