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Nucleic acid hybridization studies on mouse Friend cells
Başlık:
Nucleic acid hybridization studies on mouse Friend cells
Yazar:
Minty, Adrian J., author.
ISBN:
9780438060920
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (204 pages)
Genel Not:
Source: Dissertation Abstracts International, Volume: 76-08C.
Advisors: George Birnie.
Özet:
The Friend cell has been used to study the changes in mRNA populations which occur during differentiation and to compare the sequences present in nuclear poly(A)+ RNA with those in polysomal poly(A)+ RNA. Following treatment with DMSO, the Friend cell undergoes a number of changes which parallel those which occur during the normal maturation of erythroid cells from the proerythroblast to the orthochromatic erythroblast stage. During this differentiation there is an accumulation of globin mRNA. as well as haemoglobin, and an initial imbalance of a and p globin mRNA disappears. The rest of the mRNA. population of the Friend cell, which is shown to consist of in the order of 104 diverse mRNA species at varying abundances, remains remarkably constant following DMSO-treatment, The only marked change is the appearance of a high-abundance component which is shown to represent principally a and P globin mRNA, No new mRNA sequences are detected. The remainder of the mRNA from the untreated cell are still present at similar abundances in the DMSO-treated cell. It is shown that this does not result solely from the presence of undifferentiated cells in the DMSO-treated cell population. Since the bulk of the Friend cell mRNA sequences are also found in non-erythroid cells (a transformed mouse fibroblast cell line, mouse liver) it is concluded that the mRNA. population in differentiating Friend cells is not predominantly erythroid-specific. Only about 10% of the mRNA in the DMSO-treated Friend cell is coding for haemoglobin, and it seems likely that the specialization to haemoglobin production observed in the reticulocyte is established at a later stage in erythroid differentiation, possibly via differential mRNA stability. Some of the high abundance Friend cell mRNA are found in RITA prepared from reticulocytes but it is not clear whether this reflects the presence of contaminating nucleated-erythroid and white blood cells in the reticulocyte preparation. The sequences in nuclear poly(A)+ RNA are also relatively unchanged following DMSO-treatment of Friend cells. This MA shows a considerably higher base-sequence complexity than the polysomal poly(A)+ RNA, representing 5-10 times as many diverse RNA species. It is shown that some of this extra complexity results from RNA sequences adjacent to poly(A) in the nuclear RNA. which are not destined to become mRNAs in this cell. This nucleus-confined poly(A)+ RNA makes up about 25% of the RNA sequence adjacent to poly(A) in the steady-state nuclear RM, and constitutes about 20,000 average-size gene sequences present at about 0.5 copy per nucleus. This can be compared with the abundances of mRNA sequences, which range from about 2 to 50 copies per nucleus. mRNAs at high abundance in the polysomes are also present at high abundance in the nucleus, but the range of mRNA abundance in the nucleus is somewhat less than that in the polysomes suggesting that differential cytoplasmic metabolism may affect mRNA abundance. In an attempt to establish a coding role in other cells for the RNAs nucleus-confined in the Friend cell, the presence of these RNAs in the polysomes of 14-day mouse-embryo cells has been investigated. It appears from these experiments that some of the sequences which are nucleus-confined in the Friend cell may be present as functional mRNA in the embryonic cells, but there are a number of reasons why this conclusion can be made only tentatively. These are discussed.
Notlar:
School code: 0547
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(684860.1) | 684860-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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