Skeletal Satellite Cells, Smooth Muscle Cells, and the Inflammatory Markers Composition of Pubocervical and Rectovaginal Fibromuscularis in Women with and without Advanced Pelvic Organ Prolapse
Başlık:
Skeletal Satellite Cells, Smooth Muscle Cells, and the Inflammatory Markers Composition of Pubocervical and Rectovaginal Fibromuscularis in Women with and without Advanced Pelvic Organ Prolapse
Yazar:
Fatehchehr, Soorena, author.
ISBN:
9780438015197
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (73 pages)
Genel Not:
Source: Masters Abstracts International, Volume: 57-06M(E).
Advisors: Randy Gallucci Committee members: Jennifer Peck; Lieschen Quiroz; Abbas Shobbeiri.
Özet:
Background: Pelvic organ prolapse (POP) is defined as the descent of a pelvic organ from its normal position into the vaginal wall. POP affects numerous women's quality of life and costs billions of dollars in the United States annually. One of the major risk factors for POP is pelvic floor injury during a woman's first vaginal birth. The healing process after this injury activates a series of interactions between inflammatory and other cells in the pubocervical and rectovaginal fibromuscularis. It is unclear whether inflammatory processes are associated with pelvic floor connective tissue deficiency in women with advanced POP.
Objectives: The aim of this study is to determine whether the histologic profiles (including macrophages, dendritic cell counts, satellite cells, smooth muscle cells, and endothelial cells) of pubocervical and rectovaginal fibromuscularis differ in women with and without advanced POP.
Methods: This is a pilot cross-sectional study comparing women with and without POP who presented for surgical management to a Female Pelvic Medicine and Reconstructive Surgery (FPMRS) provider at the University of Oklahoma Health Sciences Center. Under the guidance of three-dimensional transvaginal ultrasound (3DUS), biopsies of human pubocervical and rectovaginal connective tissues were collected during surgery in an ongoing IRB-approved study. The biopsies were processed and stained with different antibody tissue markers to identify different cell populations in the pubocervical and rectovaginal fibromuscularis. Immunohistologically stained sections were imaged and imported into Image J NIH image processing software to calculate the positivity of staining cell counts per total area of biopsy samples. This was referred to as a cell counts unit. Cell counts were then used to measure the counts of macrophages, dendritic cells, satellite cells, smooth muscle cells, and endothelial cells in the tissue samples relative to different stages of the prolapse. Continuous variables were compared using Wilcoxon Rank Sum test and categorical variables with a Fisher's exact test. Distribution of continuous non ordinal variables that were non-normally distributed were compared using the Wilcoxon Rank Sum test. An alpha of 0.05 was considered significant for all statistical tests.
Results: Between July of 2013 and December of 2015, 60 patients were consented and enrolled in our study. Twenty-one samples were excluded due to inadequate amount of biopsied tissue. Thirty-nine patients were included in our study. The Stage (3-4) prolapse group was significantly older than the Stage (0-2) prolapse group. Stage (0-2) group had higher prevalence of obstetric anal injury. Otherwise, the two groups were similar. There was a significant difference in median cell counts for CD68 macrophages, CD31 endothelial cells, and CD45 immune cells in pubocervical and rectovaginal fibromuscularis cells between the Stage (3-4) prolapse group and the Stage (0-2) prolapse group. There was no significant difference between the median cell counts for CD163 M2 macrophages, CD56 smooth muscle cells, and PAX7 skeletal satellite cells from pubocervical or rectovaginal fibromuscularis samples in both prolapse groups.
Conclusions: Based on the results of this study, both smooth muscle and skeletal satellite cells are present in pubocervical and rectovaginal fibromuscularis tissues. However, there is no significant difference in the presence of smooth muscle and skeletal satellite cells between the early and advanced prolapse groups. Furthermore, the presence of inflammatory cells was detected between POP groups. There was a significant difference with similar patterns in the presence of endothelial cells, macrophages, and total inflammatory cells between early and advanced POP groups in pubocervical and rectovaginal fibromuscularis. Additionally, there was a significant difference in the presence of dendritic cells in pubocervical fibromuscularis between the early and advanced POP groups, but this difference was not significant for rectovaginal fibromuscularis tissues. Future studies are needed to confirm this finding as to more fully understand and prevent the development of prolapse throughout a women's life.
Notlar:
School code: 0361
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
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XX(691859.1) | 691859-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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