To Investigate Whether Hsp90alpha is Present on the Surface of Exosome
Başlık:
To Investigate Whether Hsp90alpha is Present on the Surface of Exosome
Yazar:
Chang, Cheng, author.
ISBN:
9780438067844
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (24 pages)
Genel Not:
Source: Masters Abstracts International, Volume: 57-06M(E).
Advisors: Wei Li Committee members: Liangyu Cheng; Qilong Ying; Ebrahim Zandi.
Özet:
Extracellular Vesicles (EVs) are composed of lipid bilayer membrane and various cargos including nucleic acids, lipids, and proteins. One type of the EVs with 50-120 nm in diameter is called exosomes, which play a critical role in cell-cell communication. Since heat shock protein-90alpha (Hsp90alpha) is secreted via exosome secretion and plays a role in tissue repair and tumorigenesis, we proposed that Hsp90alpha is present on the surface of secreted exosomes. While various exosome purification protocols have been reported, the consistency and yield of exosome isolation remained far from satisfaction. I have optimized the centrifugation protocol for exosome isolation. Specifically, I have added filtration and concentration steps after three times low-speed centrifuge to prevent unnecessary precipitation. I have quantitated the number of cells needed to get enough amount of exosome for biochemical and biological analyses of the exosome-associated Hsp90alpha. Using this optimized protocol, I have also compared parental and Hsp90alpha-knockout MDA-MB-231 cells for exosome secretion. In summary, I have made progress and much more studies are ongoing.
Notlar:
School code: 0208
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(692330.1) | 692330-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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