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Survival of a Polyextremophilic Archaeon and Function of Its Enzyme in Potentially Astrobiological Conditions
Başlık:
Survival of a Polyextremophilic Archaeon and Function of Its Enzyme in Potentially Astrobiological Conditions
Yazar:
Laye, Victoria, author.
ISBN:
9780438094093
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (74 pages)
Genel Not:
Source: Masters Abstracts International, Volume: 57-06M(E).
Advisors: Shiladitya DasSarma Committee members: Colleen Burge; Feng Chen; Russell Hill.
Özet:
Haloarchaea are extremophilic microorganisms used as models for life in niche environments on Earth, Mars, and other planets. We compared growth of Halorubrum lacusprofundi, a cold-adapted isolate from Vestfold Hills, Antarctica, with Halobacterium sp. NRC-1, a mesophilic laboratory strain. Both tolerated concentrations of magnesium and perchlorate higher than expected on Mars. These Haloarchaea were exposed to and tolerated stratospheric conditions. Psychrophilic Halorubrum has a better survival rate than Halobacterium after freeze-thaw.
Cold-active beta-galactosidase from Halorubrum was used as a model to establish effects of inhibitory conditions. The enzyme was more inhibited by magnesium. Cold-activity of the enzyme was investigated through bioinformatics, genomics, and mutagenesis by identifying divergent amino acids and creating single amino acid mutations. Importance of the residues for cold activity was tested and confirmed using steady-state kinetics. Based on this work, Halorubrum and its enzyme are ideal models for studying adaptation in polyextreme environments on Earth and elsewhere.
Notlar:
School code: 0373
Tüzel Kişi Ek Girişi:
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(693341.1) | 693341-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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