Localization of Growth Factor Binding and Activity in Embryoid Bodies Using Glycosaminoglycan Mimetics
Başlık:
Localization of Growth Factor Binding and Activity in Embryoid Bodies Using Glycosaminoglycan Mimetics
Yazar:
Laubach, Logan Keone, author.
ISBN:
9780438101388
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (53 pages)
Genel Not:
Source: Masters Abstracts International, Volume: 57-06M(E).
Advisors: Kamil Godula Committee members: Brenda Bloodgood; Aaron Coleman.
Özet:
During embryogenesis, complex balances of growth factors (GFs) influence differentiation into the three germ layers: endoderm, mesoderm, and ectoderm. Current models of development are limited to introduction of soluble factors without the ability to localize GFs to specific layers within a 3D cell scaffold. FGF2 was recruited to its cell surface receptor by glycosaminoglycan (GAG) mimetics to direct stem cell differentiation into neural rosettes. This provided the basis for testing the incorporation of these GAG mimetics into a three-dimensional (3D) scaffold, more representative of a developing embryo or organoid. Altering the structure of the GAG mimetics affected their penetration into the EBs. The longer the GAG mimetic, the larger the molecular weight and size thus taking longer to incorporate into the EB compared to a shorter GAG. No correlations were observed between the ability to penetrate EBs and the different charges of the sugars ligated to the GAG mimetics. The short GAG mimetic recruited FGF2 throughout the EBs showing their ability to localize GFs in a 3D cell scaffold. Future directions include patterning Erk phosphorylation, the first step in the signaling cascade leading to Neuroectoderm differentiation, to the localization of FGF2 by the GAG mimetics. Overall these GAG mimetics show promise as tools to further explore stem cell differentiation through the recruitment of growth factors in three dimensional cell models.
Notlar:
School code: 0033
Tüzel Kişi Ek Girişi:
Mevcut:*
Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(694148.1) | 694148-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
On Order
Liste seç
Bunu varsayılan liste yap.
Öğeler başarıyla eklendi
Öğeler eklenirken hata oldu. Lütfen tekrar deneyiniz.
:
Select An Item
Data usage warning: You will receive one text message for each title you selected.
Standard text messaging rates apply.