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Foundations of Experimentally-evolved Ionizing Radiation Resistance in Escherichia coli
Başlık:
Foundations of Experimentally-evolved Ionizing Radiation Resistance in Escherichia coli
Yazar:
Bruckbauer, Steven T., author.
ISBN:
9780355941005
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (210 pages)
Genel Not:
Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B.
Advisors: Michael M. Cox Committee members: Wesley Culberson; Richard L. Gourse; James L. Keck; David C. Schwartz; Jue D. Wang.
Özet:
Ionizing radiation (IR) is a ubiquitous stress capable of directly ionizing and indirectly oxidizing all cellular macromolecules. All organisms encode in their genome the proteins necessary to repair DNA damage caused by IR and ameliorate reactive oxygen species (ROS) formed due to radiolysis of intracellular H2O; however, the extent of damage caused by IR is such that most organisms are sensitive to irradiation. Despite the types of IR-induced damage being well-known, the determinants of whether a cell survives IR exposure are not clear. Studies of IR-resistant organisms have yielded insights into extraordinary methods of DNA repair and ROS amelioration, but there is no clear answer as to what the foundational requirements for IR resistance are. Utilizing directed evolution, we have generated populations of IR-resistant E. coli and harnessed deep-sequencing technology to determine the profile of mutations that occur in these populations. By isolating the mutations which increase IR-resistance, the evolving bacteria can then 'tell' us what alterations to existing cellular machinery can allow a cell to become IR-resistant. We are continuing to evolve four replicate populations using high energy electron beam IR in an ongoing effort to generate the most IR-resistant organisms ever produced in the laboratory. We are additionally utilizing state-of-the-art mass spectrometry technology to categorize the full profile of oxidative modifications to the proteome of E. coli. In sum, this thesis work aims to build and expand a catalogue of genetic changes that can incrementally enhance an organism's ability to withstand irradiation, and furthermore determine the nature of IR-induced protein damage in order to prevent this damage from occurring.
Notlar:
School code: 0262
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(681372.1) | 681372-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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