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Wound healing in congenic strains of mice differing in their Nramp locus
Başlık:
Wound healing in congenic strains of mice differing in their Nramp locus
Yazar:
Bryant, Mark, author.
ISBN:
9780355978445
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (268 pages)
Genel Not:
Source: Dissertation Abstracts International, Volume: 76-08C.
Özet:
Adult dermal tissue damage usually results in scar formation which can be damaging both functionally and aesthetically. The observation that fetal wounds heal without scarring has led to an interest in the basic mechanisms underlying this difference in tissue repair. Although it has been noted that fetal wounds have a much reduced inflammatory response compared to those of the adult, little is known about the role or importance of these cells in establishing the adult scar forming reaction. Previous experiments removing macrophages from the site of adult wounds showed that these cells play a role in the rate of healing. This study has investigated the wound healing response of 2 congenic strains of mice B10-L-LshR (B 10) and C57B1/10ScSn (C57) that differ in their inflammatory response to intracellular parasites, and compared their healing to a standard strain, CD1. Using routine wax histology B10 mice which have been shown to have highly activated macrophages in vitro healed quicker than the standard CD1 strain, while their congenic partner C57 mice which have low macrophage activation healed slower than CD1. Even though B10 and C57 healed at different rates the final scar as assessed macroscopically and histologically was of the same quality. In comparison to CD1 mice both B10 and C57 demonstrated reduced scarring. Further investigations using immunohistochemistry, image analysis and enzyme activity assays showed differences in their inflammatory and angiogenic response, nitric oxide production and arginase activity. These results suggest that nitric oxide may influence the rate of healing while arginase activity may affect scar quality. CNI-1493 is a tetravalent compound that inhibits cytokine inducible arginine transport and nitric oxide production in vitro and protects mice against lethal endotoxemia and carrageenan-induced inflammation. Manipulation of CD1 healing by intra-dermal injection of this inhibitor resulted in reduced scarring at 70 days post wounding compared to control wounds. Even though CNI-1493 has been shown to be a potent inhibitor of iNOS, it was found that wound nitric oxide levels were unaffected in treated wounds, while arginase levels and activity were markedly reduced. These results indicate that modulation of arginase activity may be of importance in controlling the final quality of scars.
Notlar:
School code: 1543
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(683880.1) | 683880-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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