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Molecular characterisation of a novel SEC61 homologue in Saccharomyces cerevisiae
Başlık:
Molecular characterisation of a novel SEC61 homologue in Saccharomyces cerevisiae
Yazar:
Reid, Peter J., author.
ISBN:
9780438043220
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (265 pages)
Genel Not:
Source: Dissertation Abstracts International, Volume: 76-08C.
Özet:
The budding yeast Saccharomyces cerevisiae has been used as a system to investigate a novel open reading frame identified through the Yeast Genome Sequencing Project. If expressed, this open reading frame named SSH1 (Sec Sixty one Homologue), would encode a protein bearing significant homology to the integral membrane protein Sec61p, which is a major constituent of the pore in the ER translocation machinery. Using a combination of biochemical and genetic techniques the SSH1 open reading frame has been characterised and like Sec61p, has been shown to encode a protein resident in the ER membrane, however in contrast to SEC61, SSH1 is a non-essential gene. Genetic studies have shown a strong synthetic interaction between an sshl null allele and other mutants of the protein translocation pathway accompanied with a minor defect in protein translocation into the ER. To further investigate the phenotype of cells in which Sshlp is depleted, regulated alleles of SSH1 have been constructed. In doing this a method was developed to create genomic alleles under the control of regulated promoters using a single step PCR based method. This technique has been used to replace the wild type promoters of the SEC62, CDC48 and SSH1 genes with the MET3 and GAL1 promoters. The alleles constructed have been characterised and shown to function correctly and the MET3p-SSHl allele has been used in experiments to further investigate the function of its gene product. The results obtained in this study suggest that Sshlp does play a role in ER protein translocation but this is non-essential. Sshlp may function as an alternative translocation pore in the ER or as a component of the Sec61p multimeric pore.
Notlar:
School code: 1543
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(684259.1) | 684259-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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