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The isolation of ocean microbes that produce antibiotics against Pseudomonas aeruginosa strains from cystic fibrosis patients
Başlık:
The isolation of ocean microbes that produce antibiotics against Pseudomonas aeruginosa strains from cystic fibrosis patients
Yazar:
Walsh, Dennis M., author.
ISBN:
9780438006669
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (115 pages)
Genel Not:
Source: Dissertation Abstracts International, Volume: 79-10(E), Section: B.
Advisors: Guixin Susan He.
Özet:
Microbes are the source of antibiotics. It is necessary to find new antibiotics against the bacterium Pseudomonas aeruginosa. This is the causative agent of infection in cystic fibrosis patients and it can develop antibiotic resistance.
Water samples were taken from various marine environments. With aseptic technique, 100 microl of marine water was added to each of the following microbial media; Streptomyces agar, R2A agar, Sabourauds Dextrose agar (SAB), and R2A and SAB both supplemented with sea water. The marine water was streaked for isolation, and incubated at 25°C-35°C for 3-5 days.
The cross streak method was used with all of the isolated colonies against Pseudomonas aeruginosa ATCC 39324 (PAcf), a strain isolated from a cystic fibrosis patient. If a potential antibiotic is produced by the isolated water microbe, there would be growth inhibition of PAcf near the streaked area.
Of the many tests performed only 1 isolate proved to inhibit PAcf The microbe was identified by Gas Chromatography (GC) as Pseudoalteromonas nigrifaciens. The potential antibiotics produced were acetone extracted, and identified by GC-Mass Spectrometry (GC-MS) as benzophenone, 1, 2-butanediol, and cetene. These molecules could perhaps be used in clinical phase I trials in cystic fibrosis patients. This could lead to a possible cure for this terrible disorder.
Notlar:
School code: 0111
Konu Başlığı:
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(684004.1) | 684004-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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