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Functional Analysis of Yeast Casein Kinase 22 in Vacuolar Trafficking of Candida ablicans
Başlık:
Functional Analysis of Yeast Casein Kinase 22 in Vacuolar Trafficking of Candida ablicans
Yazar:
Truong, Connie, author.
ISBN:
9780438070295
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (53 pages)
Genel Not:
Source: Masters Abstracts International, Volume: 57-06M(E).
Advisors: Robert Nissen Committee members: Edward Eivers; Hyunsook Park; Howard Xu.
Özet:
Candida albicans is an opportunistic fungal pathogen. In immunocompromised patients, it can cause a life-threatening infection. Its ability to switch morphologies from yeast to hyphae form in various environments is a key virulence trait. The regulatory pathways governing morphogenesis are extremely complex and are not fully elucidated. Our previous study found that C. albicans yeast casein kinase 2 (YCK2) plays an important role in governing morphogenesis, biofilm formation, and cell wall integrity maintenance. YCK2 is part of the casein kinase I (CK1) family. CK1 is found in most eukaryotic systems and governs various cellular processes, including cellular differentiation. Fungal CK1 homologs are also highly conserved, yet little is known about their roles in fungal systems. Saccharomyces cerevisiae YCK1 and YCK2 play redundant functions. In contrast, C. albicans YCK2 is likely the dominant CK1 homolog by which it shares similar functions to both S. cerevisiae YCK1 and YCK2. Thus, it is suggested that CK1 homologs in dimorphic fungi has acquired additional functions specific to cellular processes in morphology transition. Another CK1 homolog in C. albicans, YCK22, has significant sequence homology to S. cerevisiae YCK3 (64% identical). In S. cerevisiae, YCK3 is involved in vacuolar function and localization to the vacuolar membrane. This study investigated the roles of YCK22 in governing cell integrity, stress response, and vacuolar function by constructing a YCK22 gene deletion mutant strain and assessing the phenotypic changes affected by the loss of the YCK22 function. The results in this study showed that the yck22Delta/yck22Delta strains are similar to the wild-type in both yeast and hyphae growth condition. The growth rate, morphology, susceptibility, biofilm formation, and vacuole morphology showed no phenotypical difference when compared to the wild-type strain, unlike that seen in yck2Delta/yck2Delta . Understanding the any altered phenotype of the yck22Delta/yck22Delta mutant lacking YCK22 provides insight into the biological role of YCK22 in maintaining the fitness and pathogenesis of C. albicans.
Notlar:
School code: 0962
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(692721.1) | 692721-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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