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Operation of the Leica SP8 Multiphoton Confocal System Using Single or Multiple Fluorochromes
Başlık:
Operation of the Leica SP8 Multiphoton Confocal System Using Single or Multiple Fluorochromes
Yazar:
Rowley, Amy E., author.
ISBN:
9780438112209
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (85 pages)
Genel Not:
Source: Masters Abstracts International, Volume: 57-06M(E).
Advisors: Robert Price Committee members: Marj Pena; Jay Potts.
Özet:
The goal of this paper is to systematically examine each decision made in obtaining images on the Leica SP8 Multiphoton Confocal Microscope and discuss the basic operating parameters in order to generate publishable quality images. The capabilities of the LAS X software are discussed along with in depth dicussions of each icon in order, beginning with the Configuration icon, moving then to Acquire, and a brief overview of the possible imaging edits within the Process icon. At the conclusion of this paper, the reader should feel comfortable with the operation of a confocal system and understand the tradeoffs that occur between high quality images, specimen damage, and the time it takes to collect the image. Although this paper discusses the operation of the Leica SP8 Multiphoton Confocal Microscope specifically, the principles of confocal microscopy hold true regardless of the system hardware or software used.
Notlar:
School code: 0202
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(688897.1) | 688897-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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