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Single molecule tools. Part A, Fluorescence based approaches
Başlık:
Single molecule tools. Part A, Fluorescence based approaches
Yazar:
Walter, Nils G.
ISBN:
9780123749543
Yayın Bilgileri:
San Diego, Calif : Academic Press/Elsevier, 2010.
Fiziksel Tanımlama:
1 online resource (l, 476 pages) : illustrations (some color).
Seri:
Methods in enzymology, v. 472
Methods in enzymology ; v. 472.
İçerik:
Star polymer surface passivation for single-molecule detection / Azide-specific labeling of biomolecules by Staudinger-Bertozzi ligation: phosphine derivatives of fluorescent probes suitable for single-molecule fluorescence spectroscopy / Preparation of fluorescent pre-mRNA substrates for an smFRET study of pre-mRNA splicing in yeast / Nanovesicle trapping for studying weak protein interactions by single-molecule FRET / Droplet confinement and fluorescence measurement of single molecules / Single-molecule fluorescence spectroscopy using phospholipid bilayer nanodiscs / Single-molecule spectroscopy using microfluidic platforms / Detection of protein-protein interactions in the live cell plasma membrane by quantifying prey redistribution upon bait micropatterning / Analysis of complex single-molecule FRET time trajectories / Single-molecule fluorescence studies of intrinsically disordered proteins / Measuring the energetic coupling of tertiary contacts in RNA folding using single molecule fluorescence resonance energy transfer / A highly purified, fluorescently labeled in vitro translation system for single-molecule studies of protein synthesis / Watching individual proteins acting on single molecules of DNA / DNA curtains for high-throughput single-molecule optical imaging / Scanning FCS for the characterization of protein dynamics in live cells / Observing protein interactions and their stoichiometry in living cells by brightness analysis of fluorescence fluctuation experiments / Detection of individual endogenous RNA transcripts in situ using multiple singly labeled probes / Single mRNA tracking in live cells / Single-molecule sequencing: sequence methods to enable accurate quantitation / Real-time DNA sequencing from single polymerase molecules
Özet:
Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. This volume captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. * Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines.
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Elektronik Erişim:
ScienceDirect http://www.sciencedirect.com/science/book/9780123749543 ScienceDirect http://www.sciencedirect.com/science/bookseries/00766879/472 https://www.sciencedirect.com/science/bookseries/00766879/472Mevcut:*
Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
QP601 .M49 VOL. 472 | 1178372-1001 | Elsevier E-Kitap Koleksiyonu | Arıyor... |
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