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![Neuropeptide Detection with Cell-Based Neurotransmitter Fluorescent Engineered Reporters (CNiFERs) için kapak resmi Neuropeptide Detection with Cell-Based Neurotransmitter Fluorescent Engineered Reporters (CNiFERs) için kapak resmi](/client/assets/d79c3e4af2b6d196/ctx/images/no_image.png)
Neuropeptide Detection with Cell-Based Neurotransmitter Fluorescent Engineered Reporters (CNiFERs)
Başlık:
Neuropeptide Detection with Cell-Based Neurotransmitter Fluorescent Engineered Reporters (CNiFERs)
Yazar:
Kern, Brian Tyler, author. (orcid)0000-0002-9409-089X
ISBN:
9780438107564
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (44 pages)
Genel Not:
Source: Masters Abstracts International, Volume: 57-06M(E).
Advisors: Paul A. Slesinger Committee members: Lakshmi Devi; Venetia Zachariou.
Özet:
With the rise of the nation's opioid epidemic, there is critical need for elucidating a well understood biological mechanism of the neuronal circuitry involved in addiction. We have generated a tool that allows for real time optical detection of opioid neuropeptide release using cell based neurotransmitter fluorescent engineered reporter (CNiFER) technology expressing the delta opioid receptor. CNiFERs are human embryonic stem cells stably expressing a certain GPCR coupled to the Gq proteins, and a FRET based calcium sensor TW2B. Through in vitro characterization, the DOR CNiFER shows subnanomolar specificity for Leu-enkephalin, and a temporal resolution of seconds. This DOR CNiFER will be able to provide insight on local opioid neuropeptide activity in vivo.
Notlar:
School code: 1734
Konu Başlığı:
Tüzel Kişi Ek Girişi:
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(695427.1) | 695427-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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