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Fluoride Effects on the Growth of Oral Bacteria Streptococcus mutans, Streptococcus mitis, and Lactobacillius acidophilus and Their Protein Expressions
Başlık:
Fluoride Effects on the Growth of Oral Bacteria Streptococcus mutans, Streptococcus mitis, and Lactobacillius acidophilus and Their Protein Expressions
Yazar:
Price, Derrick Lamond, author.
ISBN:
9780438030145
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (75 pages)
Genel Not:
Source: Masters Abstracts International, Volume: 57-06M(E).
Advisors: Florence Okafor Committee members: Sampson Hopkinson; Jeanette Jones; Conwin Vanterpool.
Özet:
Fluoride is widely used in toothpaste, mouthwash, and community water treatment plants to protect users from tooth decay. Pathogenic and non-pathogenic bacteria are residents of the oral flora. Little research has shown how fluoride affects the oral flora. The objective of this study was to analyze fluoride effects on the growth and proteins expressions of select oral bacteria at different dosages of NaF. Oral bacteria S. mutans, S. mitis, and L. acidophilus are residents of the oral flora and were models for pathogenic and non-pathogenic bacteria comparison. The Bioscreen method measured the growth response of the bacteria samples at 0.125 g/mL, 0.25 g/mL, and 0.5 g/mL dosage of NaF. The results concluded that the NaF antibacterial activity affects the L. acidophilus and S. mitis bacteria growth more as compared to S. mutans. The BCA Protein Assay technique identified protein expressions of L. acidophilus, S. mutans, and. S. mitis before and after exposure to NaF. The results showed that L. acidophilus had the highest amount of protein concentration in the control but declined most after exposure to NaF as compared to S. mutans and S. mitis which were consistent with the Bioscreen results. In conclusion, this experiment has shown that NaF could possibly have a more antibacterial effect on non-pathogenic bacteria growth and protein expression as compared to the pathogenic bacteria.
Notlar:
School code: 1705
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