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Application of fluorescence staining techniques to problems in human cytogenetics
Başlık:
Application of fluorescence staining techniques to problems in human cytogenetics
Yazar:
Carlin, Eilean A., author.
ISBN:
9780438059023
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (234 pages)
Genel Not:
Source: Dissertation Abstracts International, Volume: 76-08C.
Advisors: H. A. Smith-Ferguson.
Özet:
Within the past few years new staining techniques have been developed which have enabled the accurate identification of all the chromosomes in the human karyotype. Previously identification was based mainly on morphological criteria and to a lesser extent on autoradiographic techniques. The first of these new staining methods was introduced by Caspersson and his colleagues (1969, 1970, 1971) and involved staining of metaphase spreads with the fluorochrome quinacrine mustard and viewing the preparations under UV light. Chromosomes treated in this way show a pattern of cross striations or bands. Each pair of homologues has a characteristic banding pattern by which they can be readily identified. The main purpose of this study was to assess the usefulness of the fluoroescence technique as a routine laboratory procedure and if successful to apply it to various eroblems in human cytogenetics. The more readily available and less expensive fluorochrome quinacrine dihydrochioride was used here and it was found that the staining method was simple and gave constant and reproducible results. (Abstract shortened by ProQuest.).
Notlar:
School code: 0547
Tüzel Kişi Ek Girişi:
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(684679.1) | 684679-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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