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Gene Expression Analysis during the Floral Transition in Arabidopsis thaliana FT and LTP4 Double Mutant
Başlık:
Gene Expression Analysis during the Floral Transition in Arabidopsis thaliana FT and LTP4 Double Mutant
Yazar:
Alwazna, Ahmad Osama, author.
ISBN:
9780438069022
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (86 pages)
Genel Not:
Source: Masters Abstracts International, Volume: 57-06M(E).
Advisors: Robert L. Vellanoweth Committee members: Alison McCurdy; Jamil Momand; Cecilia I. Zurita-Lopez.
Özet:
The floral transition is the process by which plants switch from the vegetative to the reproductive phase. Arabidopsis thaliana is composed of a small genome and has a rapid life cycle; therefore, it's a suitable model for our study. Under long-day photoperiods (LD), the FLOWERING LOCUS T (FT) gene is activated in the leaves, then FT protein is transported to the shoot apical meristem (SAM) where floral development is promoted. However, the exact mechanism is not clearly understood. Previous studies in our lab, revealed an increase in the lipid transfer proteins LTP3 and LTP4 expression in the leaf during the floral transition, suggesting a role for LTP3 and LTP4 as a potential flowering hormone transporter. Interestingly, when the former homologous genes were knocked down, a perennial phenotype was observed with growth resuming after senescence. To better understand the biological function of LTP4, we crossed FT mutant (ft-10) with our ltp4-RNAi generating a double mutant plant ( ft-10 ltp4). The double mutant displayed delayed flowering, up to seven and six months later compared to wild-type and ft-10, respectively. Additionally, Unusual phenotypes were observed such as bushy leaves and long bolts with few flowers at the tips. We hypothesized that LTP4 carries the flowering lipid along with FT; therefore, the exhibited phenotypes of our double mutant are due to a lack of a carrier that can move the lipid to the SAM. Here, we showed that LTP4 level is increased at the onset of bolting until 4 days after bolting (4DAB), while LTP3 peaked 4 days before bolting (4DBB) and 10DAB. Moreover, In the ltp4 mutant plants, the relative levels of the genes SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and FRUITFULL (FUL) transcripts were substantially increased after bolting in SAM and leaf, respectively.
Notlar:
School code: 0962
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(688440.1) | 688440-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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