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Gene Network Analysis Identifies a Central Posttranscriptional Regulator of Cellular Stress Survival
Başlık:
Gene Network Analysis Identifies a Central Posttranscriptional Regulator of Cellular Stress Survival
Yazar:
Tien, Matthew, author. (orcid)0000-0002-0006-9644
ISBN:
9780438083936
Yazar Ek Girişi:
Fiziksel Tanımlama:
1 electronic resource (139 pages)
Genel Not:
Source: Dissertation Abstracts International, Volume: 79-11(E), Section: B.
Advisors: Sean Crosson Committee members: Aaron R. Dinner; Tao Pan; Mike J. Rust.
Özet:
Gene regulation underlies all functional outputs of a cell. Measuring changes in gene expression during biological processes is now routine; however, understanding how complex gene regulatory responses affect cellular functions remains a challenge. To address this problem, I developed network-based strategies to interrogate publicly available gene expression data to better understand how Caulobacter crescentus survives stress. My approach identified GsrN, a conserved small RNA that is directly activated by the general stress sigma factor. I found that GsrN functions as a potent post-transcriptional regulator of survival across distinct conditions including osmotic and oxidative stress. To understand GsrN's role in stress adaptation, I developed a forward biochemical approach to identify the molecular partners of GsrN. This methodology identified the leader of katG mRNA, the sole catalase/peroxidase gene in Caulobacter crescentus, as a regulatory target of GsrN. I further demonstrated that GsrN base pairs to the leader of katG mRNA and activates expression of KatG. In summary, my dissertation outlines new computational and experimental approaches that led to new understanding of the regulation of Caulobacter crescentus stress physiology.
Notlar:
School code: 0330
Tüzel Kişi Ek Girişi:
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Yer Numarası | Demirbaş Numarası | Shelf Location | Lokasyon / Statüsü / İade Tarihi |
---|---|---|---|
XX(690445.1) | 690445-1001 | Proquest E-Tez Koleksiyonu | Arıyor... |
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